Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.
Departamento de Ciencias y Tecnología Vegetal, Campus Los Ángeles, Universidad de Concepción, Escuela de Ciencias y Tecnologías, Los Angeles, Chile.
Mycorrhiza. 2018 Oct;28(7):691-701. doi: 10.1007/s00572-018-0867-3. Epub 2018 Sep 20.
Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L dry weight and productivity increased from 10.7 to 95.8 mg L day dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.
克莱弗块菌（Terfezia claveryi Chatin）是第一种被成功栽培的荒漠块菌，其菌根植物可以通过使用荒漠块菌孢子和菌丝成功生产。然而，在可行和有利可图的情况下，使用菌丝体比孢子体更为可取。鉴于传统培养方法获得的克莱弗块菌菌丝体产量较低，因此对培养基组成进行了修改，以确定其营养需求。为此，采用 Box-Behnken 设计的响应面法进行了一项实验，以找到高产菌丝体生物量的最佳参数。最佳结果是使用葡萄糖作为碳源，培养过程中缓冲 pH 值为 5，添加维生素池，并调整 MMN 培养基中最佳碳氮源浓度。生物量产量从 0.3 增加到 3g/L 干重，生产力从 10.7 增加到 95.8mg/L 干重/天。所生产的菌丝体能够有效地定殖半日花属植物的根系，提供超过 50%的外生菌根定殖。
