固醇C-24(28)还原酶的过表达增加了酿酒酵母中麦角固醇的产量。

Overexpression of a sterol C-24(28) reductase increases ergosterol production in Saccharomyces cerevisiae.

作者信息

He Xiuping, Zhang Borun, Tan Huarong

机构信息

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China.

出版信息

Biotechnol Lett. 2003 May;25(10):773-8. doi: 10.1023/a:1023572403185.

DOI:10.1023/a:1023572403185

PMID:12882006

Abstract

Three plasmids, pHX4, pHXA4 and pHXC4, containing sterol C-24(28) reductase gene (ERG4) under the control of ERG4, ADH1 or CUP1 promoters, respectively, and the copper resistance gene as the selection marker were constructed, and they were then introduced into Saccharomyces cerevisiae. Ergosterol production in recombinant strains was enhanced. Under the optimal culture condition, ergosterol content in recombinant strains YEH56(pHX4), YEH56(pHXA4) and YEH56(pHXC4) was 1.2, 1.4 and 1.5-fold (47 mg g-1) of that in the original strain.

摘要

构建了三种质粒，即pHX4、pHXA4和pHXC4，它们分别含有在ERG4、ADH1或CUP1启动子控制下的甾醇C-24(28)还原酶基因（ERG4），并以铜抗性基因作为选择标记，然后将它们导入酿酒酵母中。重组菌株中麦角固醇的产量得到了提高。在最佳培养条件下，重组菌株YEH56(pHX4)、YEH56(pHXA4)和YEH56(pHXC4)中的麦角固醇含量分别是原始菌株的1.2倍、1.4倍和1.5倍（47 mg g-1）。