Wang Shu Fang, Chen Xing Guo, Hu Zhi De, Ju Yong
Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
Biomed Chromatogr. 2003 Jul;17(5):306-11. doi: 10.1002/bmc.247.
An efficient micellar electrokinetic capillary chromatography method was developed to analyze three major active components including morroniside, loganin and gallic acid in Fructus corni and its six preparations for the first time. The factors that could affect the separation were studied, such as the pH of the buffer, concentrations of SDS, organic modifier and beta-CD, and the applied voltage. The optimum analysis conditions were 10 mmol/L NaH(2)PO(4)-5 mmol/L Na(2)B(4)O(7) (pH 6.8) buffer containing 140 mmol/L SDS, 1 mmol/L beta-CD, 5% (v/v) methanol and 12.5 kV applied voltage. The linearity between the peak-areas and the concentrations of the analytes were investigated, and they exhibit excellent linear behavior over the concentration ranges (correlation coefficients 0.9953-0.9995). In addition, the pK(a) of gallic acid was determined using capillary zone electrophoresis. The result was consistent with that reported by the literature.
首次建立了一种高效的胶束电动毛细管色谱法，用于分析山茱萸及其六种制剂中的三种主要活性成分，包括莫诺苷、马钱苷和没食子酸。研究了可能影响分离的因素，如缓冲液的pH值、SDS、有机改性剂和β-环糊精的浓度以及施加电压。最佳分析条件为含有140 mmol/L SDS、1 mmol/Lβ-环糊精、5%（v/v）甲醇和施加电压12.5 kV的10 mmol/L NaH₂PO₄-5 mmol/L Na₂B₄O₇（pH 6.8）缓冲液。研究了峰面积与分析物浓度之间的线性关系，它们在浓度范围内表现出良好的线性行为（相关系数0.9953 - 0.9995）。此外，使用毛细管区带电泳测定了没食子酸的pKₐ。结果与文献报道一致。
