Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, 483, Exporo, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:804924. doi: 10.1155/2012/804924. Epub 2011 Aug 17.
The fruit of Cornus officinalis Sieb. et Zucc. is commonly prescribed in Asian countries as a tonic formula. In this study, the hepatoprotective effect of ethanolic extracts of the fruit of C. officinalis (ECO) was investigated in a mouse model of acetaminophen- (APAP-) induced liver injury. Pretreatment of mice with ECO (100, 250, and 500 mg/kg for 7 days) significantly prevented the APAP (200 mg/kg) induced hepatic damage as indicated by the serum marker enzymes (AST, ALT, and LDH). Parallel to these changes, ECO treatment also prevented APAP-induced oxidative stress in the mice liver by inhibiting lipid peroxidation (MDA) and restoring the levels of antioxidant enzymes (SOD, CAT, and HO-1) and glutathione. Liver injury and collagen accumulation were assessed using histological studies by hematoxylin and eosin staining. Our results indicate that ECO can prevent hepatic injuries associated with APAP-induced hepatotoxicity by preventing or alleviating oxidative stress.
山茱萸果实是亚洲国家常用的滋补配方。在这项研究中，我们研究了山茱萸果实乙醇提取物（ECO）对乙酰氨基酚（APAP）诱导的肝损伤小鼠模型的保肝作用。ECO（100、250 和 500mg/kg，预处理 7 天）预处理可显著预防 APAP（200mg/kg）诱导的肝损伤，血清标记酶（AST、ALT 和 LDH）也发生了变化。与此变化平行，ECO 处理还通过抑制脂质过氧化（MDA）和恢复抗氧化酶（SOD、CAT 和 HO-1）和谷胱甘肽水平来预防 APAP 诱导的小鼠肝脏氧化应激。肝损伤和胶原积累通过苏木精和伊红染色的组织学研究进行评估。我们的结果表明，ECO 可以通过预防或减轻氧化应激来预防与 APAP 诱导的肝毒性相关的肝损伤。
