Navet Rachel, Jarmuszkiewicz Wieslawa, Almeida Andrea Miyasaka, Sluse-Goffart Claudine, Sluse Francis E
Laboratory of Bioenergetics, Department of Life Sciences, Institute of Chemistry B6c, University of Liège, Sar-Tilman, B-4000 Liège, Belgium.
J Bioenerg Biomembr. 2003 Apr;35(2):157-68. doi: 10.1023/a:1023750204310.
Alternative oxidase (AOX) and uncoupling protein (UCP) are present simultaneously in tomato fruit mitochondria. In a previous work, it has been shown that protein expression and activity of these two energy-dissipating systems exhibit large variations during tomato fruit development and ripening on the vine. It has been suggested that AOX and UCP could be responsible for the respiration increase at the end of ripening and that the cytochrome pathway could be implicated in the climacteric respiratory burst before the onset of ripening. In this study, the use of tomato mutants that fail normal ripening because of deficiencies in ethylene perception or production as well as the treatment of one selected mutant with a chemical precursor of ethylene have revealed that the bioenergetics of tomato fruit development and ripening is under the control of this plant hormone. Indeed, the evolution pattern of bioenergetic features changes with the type of mutation and with the introduction of ethylene into an ethylene-synthesis-deficient tomato fruit mutant during its induced ripening.
交替氧化酶（AOX）和解偶联蛋白（UCP）同时存在于番茄果实线粒体中。在之前的一项研究中，已经表明这两个能量耗散系统的蛋白质表达和活性在番茄果实发育和在植株上成熟的过程中表现出很大的变化。有人提出，AOX和UCP可能是成熟末期呼吸作用增加的原因，并且细胞色素途径可能与成熟开始前的跃变型呼吸高峰有关。在本研究中，使用因乙烯感知或产生缺陷而无法正常成熟的番茄突变体，以及用乙烯化学前体处理一个选定的突变体，结果表明番茄果实发育和成熟的生物能量学受这种植物激素的控制。事实上，生物能量特征的演变模式会随着突变类型以及在乙烯合成缺陷型番茄果实突变体诱导成熟期间引入乙烯而发生变化。
