[分泌性中耳炎豚鼠咽鼓管表面活性物质的变化]

[Change of eustachian tube surfactant of guinea pigs suffering from secretory otitis media].

作者信息

Liao Zhi-su, Chen Chao, Du Han, Wu Xiu-ling, Zhu Jin-chang, Li Zhi-yuan

机构信息

Department of Otorhinolaryngology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.

出版信息

Zhonghua Er Bi Yan Hou Ke Za Zhi. 2003 Apr;38(2):101-3.

PMID:12889105

Abstract

OBJECTIVE

To observe the change of surface active agents of eustachian tube of guinea pigs suffering from secretory otitis media, to explore the effects of surface active agents on secretory otitis media.

METHODS

To establish the animal model of guinea pigs suffering from secretory otitis media. To analyse the changes of biochemical component and activity of eustachian tube's surfactant, meanwhile; to observe surfactant change after ectogenic surfactant treatment.

RESULTS

The main components of the surfactant decrease in the model group of guinea pigs suffering from secretory otitismedia, phosphatidylcholine (PC) is (19.9 +/- 1.7)%, phosphatidylethanolamine(PE) is (36.8 +/- 2.7)%, minimum surface tension (gamma min) is (18.5 +/- 2.4) mN/m, and PC is (25.7 +/- 2.1)%, PE is (43.7 +/- 3.8)%, (P < 0.001), and gamma min is (7.6 +/- 0.8) mN/m in normal group (P < 0.001). After giving Ectogenic surfactant treatment, PC and PE increase, PC is (23.3 +/- 2.2)%, PE is (42.5 +/- 3.6)%; gamma min reduce, it is (11.8 +/- 2.3) mN/m.

CONCLUSIONS

The main biochemical components of the surfactant obviously decrease, the activity of the surfactant weakens in secretory otitis media of guinea pig. This study showed the treatment of Ectogenic surfactant is effective.

摘要

目的

观察分泌性中耳炎豚鼠咽鼓管表面活性物质的变化，探讨表面活性物质对分泌性中耳炎的影响。

方法

建立分泌性中耳炎豚鼠动物模型。分析咽鼓管表面活性物质的生化成分及活性变化，同时观察外源性表面活性物质治疗后的变化。

结果

分泌性中耳炎豚鼠模型组表面活性物质的主要成分减少，磷脂酰胆碱（PC）为（19.9±1.7）%，磷脂酰乙醇胺（PE）为（36.8±2.7）%，最小表面张力（γmin）为（18.5±2.4）mN/m；正常组PC为（25.7±2.1）%，PE为（43.7±3.8）%（P<0.001），γmin为（7.6±0.8）mN/m（P<0.001）。给予外源性表面活性物质治疗后，PC和PE增加，PC为（23.3±2.2）%，PE为（42.5±3.6）%；γmin降低，为（11.8±2.3）mN/m。