Ma Zhao-xin, Chen Xu-hui, Li Ming, Huang Ping, Cao Yi, Cai Jian-feng
Department of Otorhinolaryngology, Yueyang Hospital, Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2003 Dec;38(6):445-7.
To study the effect of pulmonary surfactant on otitis media with effusion in guinea pigs to find a new way to manage otitis media with effusion.
Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pigs via a transeardrum approach to set up a model of otitis media with effusion in guinea pigs. Seven days after being injected with pulmonary surfactant (PS) by transeardrum approach, ABR threshold and histomorphological changes of eustachian tube mucosa of guinea pigs were examined by light microscopy and scanning microscopy.
Five days following inoculation of HKB serous effusion were present in the middle ear cavity of guinea pigs, but disappearance of light cone. Response (mean +/- s) threshold raised from (14.0 +/- 3.1) dB to (45.0 +/- 5.7) dB. The eustachian tube mucosa was thickened and lined eosin-stained structureless matter over mucosa, while cilia of eustachian tube mucosa irregularly arranged. Seven days after being treated by PS, serous effusion of tympanum was reduced or disappeared, and response threshold decreased from (45.0 +/- 5.7) dB to (23.5 +/- 6.3) dB. There was significantly difference between them (P < 0.001). Eustachian tube mucosa was thinned, Cilia of eustachian tube mucosa regularly arranged to the nasopharynx.
Pulmonary surfactant plays a important role in otitis media with effusion of guinea pigs.
研究肺表面活性物质对豚鼠分泌性中耳炎的作用，以寻找治疗分泌性中耳炎的新方法。
采用经鼓膜途径将灭活的肺炎球菌（HKP）溶液接种到豚鼠中耳腔，建立豚鼠分泌性中耳炎模型。经鼓膜途径注射肺表面活性物质（PS）7天后，通过光学显微镜和扫描显微镜检查豚鼠咽鼓管黏膜的听性脑干反应（ABR）阈值和组织形态学变化。
接种HKP后5天，豚鼠中耳腔出现浆液性渗出，但光锥消失。反应（均值±标准差）阈值从（14.0±3.1）dB升高到（45.0±5.7）dB。咽鼓管黏膜增厚，黏膜表面有嗜酸性无结构物质覆盖，咽鼓管黏膜纤毛排列不规则。PS治疗7天后，鼓室浆液性渗出减少或消失，反应阈值从（45.0±5.7）dB降至（23.5±6.3）dB。两者之间有显著差异（P<0.001）。咽鼓管黏膜变薄，咽鼓管黏膜纤毛向鼻咽部规则排列。
肺表面活性物质在豚鼠分泌性中耳炎中起重要作用。
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