Feldmann G, Scoazec J Y, Racine L, Bernuau D
Laboratoire de Biologie Cellulaire, INSERM U327, Faculté de Médecine Xavier-Bichat, Paris, France.
Enzyme. 1992;46(1-3):139-54. doi: 10.1159/000468781.
It is now well established that hepatocytes are the main liver cells responsible for the synthesis of plasma proteins produced by the liver. That these cells are not specialized in the production of the different plasma proteins is also well established. Presently the point still debated is whether a functional hepatocellular heterogeneity exists for plasma protein synthesis as for many other hepatocyte functions. Several physiological and pathological situations suggest that this heterogeneity takes place in the hepatocytes of two opposite hepatic lobular zones, the periportal and centrilobular zones. However, this zonal difference, which supposes different regulatory mechanisms, must be confirmed by techniques other than the now classical immunocytochemistry or the in situ hybridization technique recently proposed for the demonstration of mRNAs in hepatocytes. Another hepatocellular heterogeneity, the intercellular heterogeneity, which can be observed in the same lobular zone, is more difficult to analyze, but shows that from hepatocyte to hepatocyte a variation exists in the synthesis of a given plasma protein.
现已充分证实，肝细胞是肝脏中负责合成肝脏产生的血浆蛋白的主要细胞。同样充分证实的是，这些细胞并非专门用于产生不同的血浆蛋白。目前仍存在争议的问题是，对于血浆蛋白合成而言，是否像许多其他肝细胞功能一样存在功能性肝细胞异质性。一些生理和病理情况表明，这种异质性发生在肝小叶两个相对区域的肝细胞中，即门周区和中央区。然而，这种假设存在不同调节机制的区域差异，必须通过除目前经典的免疫细胞化学或最近提出的用于在肝细胞中显示mRNA的原位杂交技术之外的其他技术来证实。另一种肝细胞异质性，即细胞间异质性，可在同一小叶区域观察到，更难分析，但表明在给定血浆蛋白的合成中，不同肝细胞之间存在差异。
