Racine-Samson L, Scoazec J V, Moreau A, Christa L, Bernuau D, Feldmann G
Laboratoire de Biologie Celllulaire, Université Paris, France.
Histochem Cell Biol. 1996 Apr;105(4):319-29. doi: 10.1007/BF01463934.
The coexpression of normally periportal and perivenous markers has been described in heterotopically transplanted hepatocytes. To determine whether such a coexpression might also occur in hepatocytes retaining their original intrahepatic location, we compared in bile-duct-ligated livers and intrasplenically transplanted hepatocytes, the expression and distribution of the predominantly periportal glucose-phosphatase, succinate dehydrogenase, and lactate dehydrogenase, the predominantly perivenous glutamate dehydrogenase, NADPH-dehydrogenase, and beta-hydroxybutyrate dehydrogenase, and the strictly perivenous glutamine synthetase. The coexpression of high levels of the two periportal markers glucose-6-phosphatase and lactate dehydrogenase and of the perivenous marker NADPH dehydrogenase was observed in two situations: in clusters of hepatocytes isolated within the ductular proliferation in bile-duct-ligated livers and the majority of intrasplenically transplanted hepatocytes. The expression of glutamine synthetase was different according to the site. The protein was observed in certain intrasplenically transplanted hepatocytes bordering the splenic vessels but was never detected in hepatocyte clusters found in bile-duct-ligated livers. Our study therefore suggests that the coexpression of periportal and perivenous markers in the same hepatocytes is likely to be a non-specific consequence of the loss of the normal connections of hepatocytes with the normal liver microcirculation.
在异位移植的肝细胞中，已观察到通常位于汇管区和中央静脉周围的标志物的共表达。为了确定这种共表达是否也会出现在保留其原始肝内位置的肝细胞中，我们比较了胆管结扎肝脏和脾内移植肝细胞中主要位于汇管区的葡萄糖 - 磷酸酶、琥珀酸脱氢酶和乳酸脱氢酶，主要位于中央静脉周围的谷氨酸脱氢酶、NADPH - 脱氢酶和β - 羟基丁酸脱氢酶，以及严格位于中央静脉周围的谷氨酰胺合成酶的表达和分布。在两种情况下观察到高水平的两种汇管区标志物葡萄糖 - 6 - 磷酸酶和乳酸脱氢酶以及中央静脉周围标志物NADPH脱氢酶的共表达：在胆管结扎肝脏的小胆管增生内分离出的肝细胞簇中，以及大多数脾内移植的肝细胞中。谷氨酰胺合成酶的表达因部位而异。在某些与脾血管相邻的脾内移植肝细胞中观察到该蛋白，但在胆管结扎肝脏中发现的肝细胞簇中从未检测到。因此，我们的研究表明，同一肝细胞中汇管区和中央静脉周围标志物的共表达可能是肝细胞与正常肝脏微循环正常连接丧失的非特异性结果。
