Bernuau D, Poliard A, Tournier I, Sala-Trepat J, Feldmann G
Cell Biol Int Rep. 1985 Jan;9(1):31-42. doi: 10.1016/0309-1651(85)90139-0.
Immunoperoxidase techniques have yielded conflicting results concerning the percentage of hepatocytes engaged in albumin production in normal adult rats. In addition, the question of whether functional differences in the synthesis of plasma proteins exist within the hepatic lobule remains to be determined. To clarify these questions, we have searched for gene albumin transcripts by in situ hybridization, and for the corresponding protein by immunoperoxidase, on adjacent liver sections. We observed that all hepatocytes contain albumin transcripts as well as the albumin protein, without any detectable zonal variation within the liver lobule. Taken altogether, these results demonstrate that every hepatocyte, whatever its location in the hepatic lobule, is actively engaged in albumin gene expression.
免疫过氧化物酶技术在正常成年大鼠中，关于参与白蛋白产生的肝细胞百分比得出了相互矛盾的结果。此外，肝小叶内血浆蛋白合成是否存在功能差异的问题仍有待确定。为了阐明这些问题，我们通过原位杂交在相邻的肝脏切片上寻找白蛋白基因转录本，并通过免疫过氧化物酶寻找相应的蛋白质。我们观察到所有肝细胞都含有白蛋白转录本以及白蛋白蛋白，在肝小叶内没有任何可检测到的区域差异。综上所述，这些结果表明，无论在肝小叶中的位置如何，每个肝细胞都积极参与白蛋白基因表达。
