Dua H S, Reyes P F, Barrett J A, Abrams M S, Schwarting R, Craft C M, Donoso L A
Retina Service, Wills Eye Hospital, Philadelphia, PA 19107.
Eye (Lond). 1992;6 ( Pt 6):594-8. doi: 10.1038/eye.1992.128.
Sensitisation to retinal S-antigen has been implicated in the pathogenesis of several clinical forms of posterior uveitis. S-antigen-like molecules have recently been demonstrated in the brain and choroid plexus of experimental animals. We used a panel of four monoclonal antibodies (MAbs), MAbF4-C1, MAbC10-C10, MAbA2-G5 and MAbA9-C6, which define specific epitopes in the amino, mid and carboxyl terminal portions of S-antigen in order to identify an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid (CSF). Three MAbs, MAbF4-C1, MAbC10-C10 and MAbA9-C6, localised an S-antigen-like molecule to the cytoplasm of the epithelial cells of the human choroid plexus. Polymerase chain reaction of cDNA from choroid plexus verified the presence of S-antigen homologues in the choroid plexus. The presence of an S-antigen-like molecule in the CSF was demonstrated by western blots in seven CSF samples from patients with a variety of neuropathological disorders. It is proposed that immunological cross-reactivity and biochemical similarity between retinal S-antigen and an S-antigen-like molecule in human choroid plexus and CSF could form a basis for neurological manifestations observed in certain clinical forms of uveitides.
视网膜S抗原致敏与几种后葡萄膜炎临床类型的发病机制有关。最近在实验动物的脑和脉络丛中发现了S抗原样分子。我们使用一组四种单克隆抗体（MAb），即MAbF4-C1、MAbC10-C10、MAbA2-G5和MAbA9-C6，它们可识别S抗原氨基、中部和羧基末端部分的特定表位，以鉴定人脉络丛和脑脊液（CSF）中的一种S抗原样分子。三种单克隆抗体，MAbF4-C1、MAbC10-C10和MAbA9-C6，将一种S抗原样分子定位于人脉络丛上皮细胞的细胞质中。脉络丛cDNA的聚合酶链反应证实脉络丛中存在S抗原同源物。通过蛋白质印迹法在来自患有各种神经病理学疾病患者的7份脑脊液样本中证实了脑脊液中存在S抗原样分子。有人提出，视网膜S抗原与人脉络丛和脑脊液中的S抗原样分子之间的免疫交叉反应性和生化相似性可能是某些葡萄膜炎临床类型中观察到的神经学表现的基础。
