Lieb W E, Smith-Lang L, Dua H S, Christensen A C, Donoso L A
Retina Service, Wills Eye Hospital, Philadelphia, PA 19107.
Exp Eye Res. 1991 Aug;53(2):171-8. doi: 10.1016/0014-4835(91)90071-l.
Vision is a highly complex process common to many vertebrate and invertebrate species. The visual system of Drosophila represents a valuable model in which to study this process since its chromosomal makeup has been well mapped and many mutants containing specific photoreceptor defects have been described. S-antigen is a well-characterized photoreceptor cell protein which plays a fundamental role in the visual process. In this study, we used a panel of four monoclonal antibodies (MAbs) specific for amino, mid and carboxy terminal amino acid sequences in S-antigen in order to examine S-antigen immunoreactivity in the Drosophila visual system. Two MAbs, MAb5C6.47 and MAbC10-C10, localized S-antigen to the retinules and ocelli. In contrast, MAbA2-G5 and MAbA9-C6 did not stain. The presence and strong antigenic similarity of an S-antigen homolog in Drosophila suggests that in both vertebrates and invertebrates S-antigen plays a major role in the regulation of the phototransduction of vision.
视觉是许多脊椎动物和无脊椎动物共有的高度复杂的过程。果蝇的视觉系统是研究这一过程的宝贵模型，因为其染色体组成已被精确绘制，并且已经描述了许多含有特定光感受器缺陷的突变体。S抗原是一种特征明确的光感受器细胞蛋白，在视觉过程中起基本作用。在本研究中，我们使用了一组针对S抗原氨基、中部和羧基末端氨基酸序列的四种单克隆抗体（MAb），以检测果蝇视觉系统中的S抗原免疫反应性。两种MAb，MAb5C6.47和MAbC10-C10，将S抗原定位到小眼和单眼中。相比之下，MAbA2-G5和MAbA9-C6没有染色。果蝇中S抗原同源物的存在及其强烈的抗原相似性表明，在脊椎动物和无脊椎动物中，S抗原在视觉光转导的调节中都起主要作用。
