Maksimov V F, Kolpakov A R, Panin L E, Korostyshevskaia I M, Shalbueva N I
Institute of Biochemistry, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk.
Morfologiia. 2003;123(2):56-60.
The distribution of different classes of colloidal gold-labeled lipoproteins (LP) was studied at the ultrastructural level in rat myocardium using the isolated heart perfusion following the adrenaline stimulation. The distribution of label was examined in endotheliocytes, macrophages and in the interstitial space. The addition of adrenaline to Krebs solution during 30 min of heart perfusion in vitro was shown to increase the amount, but to decrease the velocity of penetration of labeled LP of very low and low density into the myocardium. The intensive utilization of lipid component from LP in the conditions of stimulation provides for the enhanced metabolic requirements of the cardiomyocytes and good preservation of their ultrastructure. It is possible that colloidal gold-associated protein component of LP is incorporated by the endothelium more slowly. The label penetrates into the interstitium and is accumulated in the activated macrophages. If high density LP are added together with adrenaline into the perfusion medium the label does not leave the capillary wall, while macrophages and cardiomyocytes acquire the ultrastructural features of high functional activity.
在肾上腺素刺激后，利用离体心脏灌注技术，在超微结构水平上研究了大鼠心肌中不同类别的胶体金标记脂蛋白（LP）的分布。在内皮细胞、巨噬细胞和间质空间中检测了标记物的分布。体外心脏灌注30分钟期间，向 Krebs 溶液中添加肾上腺素可增加极低密度和低密度标记LP进入心肌的量，但降低其渗透速度。在刺激条件下，LP脂质成分的大量利用满足了心肌细胞增强的代谢需求，并使其超微结构得到良好保存。LP与胶体金结合的蛋白质成分可能被内皮细胞摄取得更慢。标记物渗透到间质中并积聚在活化的巨噬细胞中。如果将高密度LP与肾上腺素一起添加到灌注介质中，标记物不会离开毛细血管壁，而巨噬细胞和心肌细胞则呈现出高功能活性的超微结构特征。
