Panin L E, Poliakov L M, Voĭtsekhovskaia E E, Bikkina M M
Tsitologiia. 1981 Nov;23(11):1257-62.
One hour incubation of slices prepared from rat liver or adrenal glands with 125I labeled lipoproteins of different classes resulted in label binding and its penetration into the tissue cells. The greatest amounts of labeling were found in fractions of mitochondria, lysosomes, microsomes and the final supernatant. In relation to the weight unit, slices from adrenal glands consumed several times as much label as did liver slices. Epinephrine increased the amount of the label bound to the low density lipoproteins by several times in the fractions of large cell membranes, and decreased the 125I level in mitochondria. Cortizole increased the incorporation of the protein labeled lipoproteins very low density into the liver mitochondria. ACTH did not influence the incorporation or cellular distribution of the label in adrenal glands.
用不同类别的125I标记脂蛋白对大鼠肝脏或肾上腺制备的切片进行一小时孵育，导致标记物结合并渗透到组织细胞中。在线粒体、溶酶体、微粒体和最终上清液部分发现了最大量的标记。以重量单位计，肾上腺切片消耗的标记物是肝脏切片的几倍。肾上腺素使大细胞膜部分中与低密度脂蛋白结合的标记物量增加了几倍，并降低了线粒体中的125I水平。皮质醇增加了极低密度蛋白标记脂蛋白进入肝脏线粒体的掺入量。促肾上腺皮质激素不影响肾上腺中标记物的掺入或细胞分布。
