Isik K, Chun J, Hah Y C, Goodfellow M
Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, UK.
Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1227-30. doi: 10.1099/00207713-49-3-1227.
A soil isolate representing the putatively novel species 'Nocardia uniformis' was found to have morphological, staining and chemotaxonomic properties consistent with its classification in the genus Nocardia. An almost complete sequence of the 16S rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organism was consistently associated with the type strain of Nocardia otitidiscaviarum albeit with a relatively low bootstrap value recorded for neighbour-joining analysis. The strain was also readily separated from representatives of all validly described Nocardia species using a set of phenotypic properties. The genotypic and phenotypic data indicate that the strain should be assigned to the genus Nocardia as a new species. The name proposed for the new species is Nocardia uniformis. The type strain is JCM 3224T.
一种被认为代表新物种“均匀诺卡氏菌”的土壤分离株，其形态、染色和化学分类学特性与其在诺卡氏菌属中的分类一致。通过对扩增基因进行克隆和测序，确定了该菌株16S rDNA的几乎完整序列。将该序列与诺卡氏菌的可用序列进行比对，并使用四种建树算法推断系统发育树。尽管邻接法分析记录的自展值相对较低，但该菌株始终与耳腔诺卡氏菌模式菌株相关联。利用一组表型特性，该菌株也很容易与所有已有效描述的诺卡氏菌属物种的代表区分开来。基因型和表型数据表明，该菌株应作为一个新物种归入诺卡氏菌属。为该新物种提议的名称是均匀诺卡氏菌。模式菌株是JCM 3224T。
