Hall Val, Collins Matthew D, Hutson Roger A, Lawson Paul A, Falsen Enevold, Duerden Brian I
Anaerobe Reference Unit, PHLS, University Hospital of Wales, Cardiff CF14 4XW, UK.
School of Food Biosciences, University of Reading, Reading, UK.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1065-1068. doi: 10.1099/ijs.0.02442-0.
An unusual gram-positive, facultatively anaerobic, catalase-positive, diphtheroid-shaped organism originating from an unknown human clinical source was characterized by biochemical, molecular chemical and molecular phylogenetic methods. Based on its morphological and biochemical characteristics and the presence of a murein based on meso-diaminopimelic acid, the unidentified organism was tentatively assigned to the genus Corynebacterium. However, the unknown organism was found to lack the distinctive, short-chain corynomycolic acids that are considered to be characteristic of this genus. Despite the absence of these characteristic lipids, comparative 16S rRNA gene sequencing showed that the unknown bacterium was phylogenetically a member of the genus Corynebacterium and was distinct from all currently known species. Based on both phenotypic and 16S rRNA sequence considerations, it is proposed that the unknown organism be classified as a novel species, Corynebacterium atypicum sp. nov. The type strain of C. atypicum is strain R2070T (=CCUG 45804T=CIP 107431T).
一株源自不明人类临床样本的罕见革兰氏阳性、兼性厌氧、过氧化氢酶阳性、类白喉杆菌形态的微生物，通过生化、分子化学和分子系统发育方法进行了表征。基于其形态和生化特征以及基于内消旋二氨基庚二酸的胞壁质的存在，该未鉴定的微生物被初步归为棒状杆菌属。然而，发现该未知微生物缺乏被认为是该属特征的独特短链分枝菌酸。尽管没有这些特征性脂质，但比较16S rRNA基因测序表明，该未知细菌在系统发育上是棒状杆菌属的成员，并且与所有目前已知的物种不同。基于表型和16S rRNA序列的考虑，建议将该未知微生物分类为一个新物种，非典型棒状杆菌（Corynebacterium atypicum sp. nov.）。非典型棒状杆菌的模式菌株是R2070T（=CCUG 45804T=CIP 107431T）。
