Fernández-Garayzábal J F, Egido R, Vela A I, Briones V, Collins M D, Mateos A, Hutson R A, Domínguez L, Goyache J
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
School of Food Biosciences, University of Reading, Reading RG6 6AP, UK.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1135-1138. doi: 10.1099/ijs.0.02533-0.
Biochemical, molecular chemical and molecular genetic studies were performed on seven unidentified gram-positive, rod-shaped organisms recovered from eagles. The strains were provisionally identified as Corynebacterium jeikeium with the commercial API Coryne system, but they were able to grow under anaerobic conditions and were non-lipophilic. Comparative 16S rRNA gene sequencing studies demonstrated that the isolates belonged phylogenetically to the genus Corynebacterium. Three strains were identified genotypically as Corynebacterium falsenii; the remaining four strains corresponded to a hitherto unknown lineage within the genus Corynebacterium, associated with a small subcluster of species that included Corynebacterium diphtheriae and its close relatives. The unknown bacterial strains were readily distinguished from these and other species of the genus by biochemical tests. Based on both phenotypic and phylogenetic evidence, it is proposed that the unknown bacterial strains from eagles should be classified as Corynebacterium aquilae sp. nov. (type strain is S-613T = CECT 5993T = CCUG 46511T).
对从鹰身上分离出的7株未鉴定的革兰氏阳性杆状菌进行了生化、分子化学和分子遗传学研究。这些菌株用商业API Coryne系统初步鉴定为杰氏棒状杆菌，但它们能够在厌氧条件下生长且不嗜脂。比较16S rRNA基因测序研究表明，这些分离株在系统发育上属于棒状杆菌属。3株菌株在基因型上被鉴定为法氏棒状杆菌；其余4株菌株对应于棒状杆菌属内一个迄今未知的谱系，与包括白喉棒状杆菌及其近缘种在内的一小类物种相关。通过生化试验，这些未知细菌菌株很容易与该属的其他物种区分开来。基于表型和系统发育证据，建议将来自鹰的未知细菌菌株分类为新种鹰棒状杆菌（模式菌株为S-613T = CECT 5993T = CCUG 46511T）。
