Sebastiani G D, Galeazzi M, Tincani A, Scorza R, Mathieu A, Passiu G, Morozzi G, Piette J C, Cervera R, Houssiau F, Smolen J, Fernandez Nebro A, De Ramon E, Goral A Jedryka, Papasteriades C, Ferrara G B, Carcassi C, Bellisai F, Marcolongo R
UO Complessa di Reumatologia, Azienda Ospedaliera San Camillo-Forlanini, Roma, Italy.
Lupus. 2003;12(7):560-3. doi: 10.1191/0961203303lu402oa.
Our objective was to determine the HLA-DPB1 allele associations of anticardiolipin (aCL) and anti-beta2GPI (a(beta)2GPI) antibodies, and of clinical manifestations of the antiphospholipid syndrome (APS), in systemic lupus erythematosus (SLE). We studied 577 European patients with SLE. aCL and a(beta)2GPI antibodies were measured by ELISA. Molecular typing of HLA-DPB1 locus was performed by polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) method. aCL showed positive association with -DPB11501 (P = 0.005, OR = 7.4), and -DPB12301 (P = 0.009, OR = 3.3). a(beta)2GPI showed positive association with -DPB10301 (P = 0.01, OR = 1.9), and -DPB11901 (P = 0.004, OR = 8.1). In addition, livedo reticularis was associated with -DPB11401, and Raynaud's phenomenon with -DPB12001. In conclusion, HLA-DPB1 locus may contribute to the genetic predisposition to develop antiphospholipid antibodies and clinical manifestations of the APS in patients with SLE.
我们的目的是确定系统性红斑狼疮（SLE）患者中抗心磷脂（aCL）和抗β2糖蛋白I（aβ2GPI）抗体的HLA - DPB1等位基因关联，以及抗磷脂综合征（APS）的临床表现。我们研究了577名欧洲SLE患者。通过酶联免疫吸附测定法（ELISA）检测aCL和aβ2GPI抗体。采用聚合酶链反应 - 序列特异性寡核苷酸探针（PCR - SSOP）方法对HLA - DPB1基因座进行分子分型。aCL与 - DPB11501呈正相关（P = 0.005，OR = 7.4），与 - DPB12301呈正相关（P = 0.009，OR = 3.3）。aβ2GPI与 - DPB10301呈正相关（P = 0.01，OR = 1.9），与 - DPB11901呈正相关（P = 0.004，OR = 8.1）。此外，网状青斑与 - DPB11401相关，雷诺现象与 - DPB12001相关。总之，HLA - DPB1基因座可能有助于SLE患者发生抗磷脂抗体和APS临床表现的遗传易感性。
