Lazarow Paul B
Mount Sinai School of Medicine, 1190 Fifth Avenue, Box 1007, New York, NY 10029-6574, USA.
Curr Opin Cell Biol. 2003 Aug;15(4):489-97. doi: 10.1016/s0955-0674(03)00082-6.
Investigations of peroxisome biogenesis in diverse organisms reveal new details of this unique process and its evolutionary conservation. Interactions among soluble receptors and the membrane peroxins that catalyze protein translocation are being mapped. Ubiquitination is observed. A receptor enters the organelle carrying folded cargo and recycles back to the cytosol. Tiny peroxisome remnants - vesicles and tubules - are discovered in pex3 mutants that lack the organelle. When the mutant is transfected with a good PEX3 gene, these protoperoxisomes acquire additional membrane peroxins and then import the matrix enzymes to reform peroxisomes. Thus, de novo formation need not be postulated. Dynamic imaging of yeast reveals dynamin-dependent peroxisome division and regulated actin-dependent segregation of the organelle before cell division. These results are consistent with biogenesis by growth and division of pre-existing peroxisomes.
对多种生物体中过氧化物酶体生物发生的研究揭示了这一独特过程及其进化保守性的新细节。正在绘制可溶性受体与催化蛋白质转运的膜过氧化物酶之间的相互作用图谱。观察到泛素化现象。一种受体携带折叠好的货物进入细胞器，然后循环回到细胞质溶胶。在缺乏该细胞器的pex3突变体中发现了微小的过氧化物酶体残余物——囊泡和小管。当用良好的PEX3基因转染该突变体时，这些原过氧化物酶体获得额外的膜过氧化物酶，然后导入基质酶以重新形成过氧化物酶体。因此，无需假定从头形成。酵母的动态成像揭示了动力蛋白依赖性的过氧化物酶体分裂以及细胞分裂前该细胞器受调控的肌动蛋白依赖性分离。这些结果与通过预先存在的过氧化物酶体的生长和分裂进行生物发生相一致。
