Zhao Yunde, Dai Xinhua, Blackwell Helen E, Schreiber Stuart L, Chory Joanne
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116, USA.
Science. 2003 Aug 22;301(5636):1107-10. doi: 10.1126/science.1084161. Epub 2003 Jul 31.
Auxin is a plant hormone that regulates many aspects of plant growth and development. We used a chemical genetics approach to identify SIR1, a regulator of many auxin-inducible genes. The sir1 mutant was resistant to sirtinol, a small molecule that activates many auxin-inducible genes and promotes auxin-related developmental phenotypes. SIR1 is predicted to encode a protein composed of a ubiquitin-activating enzyme E1-like domain and a Rhodanese-like domain homologous to that of prolyl isomerase. We suggest a molecular context for how the auxin signal is propagated to exert its biological effects.
生长素是一种植物激素，可调节植物生长和发育的许多方面。我们采用化学遗传学方法鉴定出SIR1，它是许多生长素诱导基因的调节因子。sir1突变体对sirtinol具有抗性，sirtinol是一种能激活许多生长素诱导基因并促进生长素相关发育表型的小分子。预计SIR1编码一种蛋白质，该蛋白质由一个泛素激活酶E1样结构域和一个与脯氨酰异构酶类似的硫氧还蛋白样结构域组成。我们提出了一个关于生长素信号如何传播以发挥其生物学效应的分子背景。
