Huang Yung-Chieh, Chang Yueh-Long, Hsu Jen-Jen, Chuang Huey-wen
Department of BioAgricultural Sciences, National Chiayi University, Chiayi, Taiwan.
Gene. 2008 Sep 1;420(2):118-24. doi: 10.1016/j.gene.2008.05.017. Epub 2008 Jun 4.
Plant hormone auxin elicits diverse responses in plant growth and development. Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a comparative transcriptome analysis of auxin responsive genes between Arabidopsis Columbia ecotype and the auxin insensitive mutant eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. This study demonstrates that auxin exerts its effect in complex transcriptional networks.
植物激素生长素在植物生长发育过程中引发多种反应。积累的数据表明，泛素介导的蛋白水解途径在生长素信号转导中起关键作用。为了更深入了解生长素作用的分子机制，我们通过cDNA-AFLP对拟南芥哥伦比亚生态型和生长素不敏感突变体eta2之间的生长素响应基因进行了比较转录组分析。使用256种引物组合，扩增出约5900个转录本衍生片段（TDF）。通过DNA斑点印迹分析确认了66个差异表达的TDF。序列分析表明，大量参与转录调控或RNA代谢的基因被鉴定为生长素调控基因。Northern印迹分析证实了16个生长素调控基因的转录水平。这些基因包括各种形式的转录调节因子、防御相关基因、RING型泛素连接酶和糖基水解酶。本研究表明，生长素在复杂的转录网络中发挥作用。
