Darouez Hajer, Werbrouck Stefaan P O
Laboratory for Applied In Vitro Plant Biotechnology, Ghent University, 9000 Ghent, Belgium.
Plants (Basel). 2025 Jan 2;14(1):108. doi: 10.3390/plants14010108.
Efficient adventitious root formation is essential in micropropagation. Auxin prodrugs, inactive precursors that convert into active auxins within the plant, offer potentially improved rooting control and reduced phytotoxicity. This study investigated the efficacy of dichlorprop ester (DCPE), commercialized as Corasil and Clemensgros (originally intended to increase grapefruit size), in promoting in vitro root initiation in the model plant × , compared to its hydrolyzed form DCP and the related compound C77. DCPE displayed a stronger root-inducing effect than DCP, especially at lower concentrations (0.01 and 0.1 µM). Notably, at 1 µM, both DCP and DCPE induced abundant aerial root formation, a phenomenon not previously observed in poplar with traditional auxin treatments. Metabolite analysis revealed distinct patterns. DCPE treatment resulted in rapid hydrolysis to DCP, leading to faster and more systemic distribution of the active auxin throughout the plant, compared to direct DCP application. C77 treatments showed slower uptake and limited translocation combined with slow metabolism to DCP. These results highlight the potential of auxin prodrugs like DCPE as an effective and controllable auxin source for optimizing in vitro rooting protocols in woody plant species.
在微繁殖中，高效的不定根形成至关重要。生长素前体药物是在植物体内转化为活性生长素的无活性前体，有望改善生根控制并降低植物毒性。本研究调查了以Corasil和Clemensgros商业化的二氯丙酸酯（DCPE，最初旨在增加葡萄柚大小）与水解形式的DCP和相关化合物C77相比，在模式植物×中促进体外生根的功效。DCPE表现出比DCP更强的生根诱导作用，尤其是在较低浓度（0.01和0.1μM）下。值得注意的是，在1μM时，DCP和DCPE均诱导大量气生根形成，这是传统生长素处理杨树中以前未观察到的现象。代谢物分析揭示了不同的模式。与直接施用DCP相比，DCPE处理导致快速水解为DCP，从而使活性生长素在植物体内分布更快、更系统。C77处理显示吸收较慢且转运有限，同时代谢为DCP的速度也较慢。这些结果突出了DCPE等生长素前体药物作为有效且可控的生长素来源，用于优化木本植物物种体外生根方案的潜力。
