Heymer J, Marquardt J, Heinz H, Adolf G R, Leibold W
Immunology Unit, Veterinary School, Hannover, FRG.
J Interferon Res. 1992 Dec;12(6):415-23. doi: 10.1089/jir.1992.12.415.
The influence of recombinant equine interferon-beta 1 (rEqIFN-beta 1) on mononuclear cells of peripheral blood (PBMC) and polymorphonuclear neutrophilic granulocytes (PMN) was tested under in vitro and ex vivo conditions. Treatment of equine PBMC with IFN in vitro enhanced the antibody-independent cytotoxicity (AICC) and antibody-dependent cytotoxicity (ADCC) while there was no significant effect on the cytotoxic capacity of PMN treated with rEqIFN-beta 1 in vitro. Ex vivo there was an increased capacity of AICC and ADCC upon single or multiple application of rEqIFN-beta 1 in PMN, only. Treatment with rEqIFN-beta 1 thus induced an increased cellular cytotoxicity in vitro and in vivo but in different populations of peripheral blood cells. In vivo rEqIFN-beta 1 causes a pronounced activation of PMN but not of PBMC as cytotoxic effector cells. This might be achieved indirectly, e.g., by cytokines produced by IFN-sensitive cells.
在体外和体内条件下测试了重组马β-干扰素1（rEqIFN-β1）对外周血单个核细胞（PBMC）和多形核嗜中性粒细胞（PMN）的影响。体外使用IFN处理马PBMC可增强抗体非依赖性细胞毒性（AICC）和抗体依赖性细胞毒性（ADCC），而体外使用rEqIFN-β1处理PMN的细胞毒性能力无显著影响。在体内，仅在PMN中单次或多次应用rEqIFN-β1后，AICC和ADCC的能力增强。因此，rEqIFN-β1处理在体外和体内均可诱导细胞毒性增加，但作用于外周血不同细胞群体。在体内，rEqIFN-β1可使PMN作为细胞毒性效应细胞发生明显激活，但不会激活PBMC。这可能是通过IFN敏感细胞产生的细胞因子间接实现的。
