Heinz H, Marquardt J, Schuberth H J, Adolf G R, Leibold W
Immunology Unit, Veterinary School, Hannover, Germany.
Vet Immunol Immunopathol. 1994 Sep;42(3-4):221-35. doi: 10.1016/0165-2427(94)90069-8.
Peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophilic granulocytes (PMN) as well as embryonic equine dermal fibroblasts and the equine fibroblast line E. Derm which were used as controls, were treated with recombinant equine interferon-beta 1 (rEqIFN-beta 1) in vitro which induced the expression of different proteins in these cells. A 74 kDa protein was induced in PBMC and an 82 kDa protein was additionally found in the equine fibroblast E. Derm cell line following treatment with rEqFN-beta 1. Both proteins reacted with anti-mouse and anti-human Mx protein antisera in immunoblot tests. The 74 kDa and perhaps the 82 kDa components may thus represent equine 'Mxanalogous proteins'. The 74 kDa protein was only detected in PBMC of ten out of 20 horses examined. The induction of Mx protein in the horse by Type 1 interferon may therefore resemble that in the mouse, where Mx protein is involved in selective resistance to influenza virus. The influence of rEqIFN-beta 1 on protein expression in equine PBMC and PMN was monitored by metabolic labeling and 2-D gel electrophoresis. Proteins of 82, 74, 58 and 40 kDa were induced in PBMC following exposure to rEqIFN-beta 1. A constitutively expressed 35 kDa protein, however, was no longer demonstrable upon treatment with interferon. None of the proteins induced within PBMC was found in highly purified PMN treated with interferon. PMN exposed to rEqIFN-beta 1 synthesized four proteins in the range of 25 to 27 kDa. These proteins have not been described in interferon-treated PMN of any other species.
外周血单个核细胞（PBMC）、多形核嗜中性粒细胞（PMN）以及用作对照的胚胎马皮肤成纤维细胞和马成纤维细胞系E. Derm，在体外接受重组马干扰素-β1（rEqIFN-β1）处理，这诱导了这些细胞中不同蛋白质的表达。在用rEqFN-β1处理后，PBMC中诱导出一种74 kDa的蛋白质，在马成纤维细胞E. Derm细胞系中还额外发现了一种82 kDa的蛋白质。在免疫印迹试验中，这两种蛋白质均与抗小鼠和抗人Mx蛋白抗血清发生反应。因此，74 kDa以及可能的82 kDa成分可能代表马的“Mx类似蛋白”。在所检测的20匹马中，仅在10匹马的PBMC中检测到了74 kDa的蛋白质。因此，1型干扰素在马中诱导Mx蛋白的情况可能与在小鼠中相似，在小鼠中Mx蛋白参与对流感病毒的选择性抗性。通过代谢标记和二维凝胶电泳监测rEqIFN-β1对马PBMC和PMN中蛋白质表达的影响。暴露于rEqIFN-β1后，PBMC中诱导出了82、74、58和40 kDa的蛋白质。然而，一种组成性表达的35 kDa蛋白质在用干扰素处理后不再能检测到。在用干扰素处理的高度纯化的PMN中未发现PBMC中诱导出的任何蛋白质。用rEqIFN-β1处理的PMN合成了四种分子量在25至27 kDa范围内的蛋白质。在任何其他物种经干扰素处理的PMN中均未描述过这些蛋白质。
