一种以重组马β-1干扰素为例检测“难检测”干扰素的方法。
A method for the assay of "difficult" interferons exemplified with recombinant equine interferon-beta 1.
作者信息
Marquardt J, Heinz H, Heymer J, Deegen E, Adolf G R, Leibold W
机构信息
Immunology Unit, Veterinary School, Hannover, FRG.
出版信息
J Interferon Res. 1992 Apr;12(2):83-5. doi: 10.1089/jir.1992.12.83.
We wished to assay recombinant equine interferon-beta 1 (rEqIFN-beta 1) but could not obtain satisfactory results with previously described methods. Therefore, we developed a yield-reduction assay, using primary horse peripheral blood mononuclear cells (PBMC) with vesicular stomatitis virus (VSV) for challenge, which proved consistently satisfactory and highly sensitive. It is suggested that this method of assay may be useful for IFNs from other animals where problems are encountered.
我们希望检测重组马干扰素-β1（rEqIFN-β1），但使用先前描述的方法未能获得满意的结果。因此，我们开发了一种产量降低检测法，使用原代马外周血单核细胞（PBMC）并用水疱性口炎病毒（VSV）进行攻击，结果证明该方法一直令人满意且高度灵敏。建议这种检测方法可能对在检测其他动物干扰素时遇到问题的情况有用。
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