Nano Rosanna, Balegno Sabrina, Vaccarone Rita, Corato Manuel, Ceroni Mauro
Department of Animal Biology, University of Pavia, IGM-CNR, Department of Histochemistry and Cytochemistry, Pavia, Italy.
Anticancer Res. 2003 May-Jun;23(3B):2377-81.
Different immunohistochemical techniques have been employed to identify the anti-neuronal antibodies in patients with paraneoplastic neurological syndromes. The finding of anti-neuronal-specific autoantibodies in serum or cerebrospinal fluid well correlates with particular types of tumors, thus leading, in many cases, to an early cancer identification. In this work, we have compared three immunohistochemical methods (immunofluorescence, indirect immunoperoxidase and avidin-biotin immunoperoxidase) on frozen and paraffin-embedded sections of rat cerebellum in order to set up a reliable and simple technique for the diagnosis of these syndromes. Our study demonstrates that the best result was obtained by using frozen sections of rat cerebellum and the avidin-biotin immunoperoxidase method that also allowed the identification of anti-GAD antibodies not detected in paraffin-embedded tissues.
已采用不同的免疫组织化学技术来鉴定副肿瘤性神经系统综合征患者体内的抗神经元抗体。在血清或脑脊液中发现抗神经元特异性自身抗体与特定类型的肿瘤密切相关，因此在许多情况下可实现癌症的早期诊断。在本研究中，我们比较了三种免疫组织化学方法（免疫荧光法、间接免疫过氧化物酶法和抗生物素蛋白-生物素免疫过氧化物酶法），用于大鼠小脑冰冻切片和石蜡包埋切片，以建立一种可靠且简便的技术用于诊断这些综合征。我们的研究表明，使用大鼠小脑冰冻切片和抗生物素蛋白-生物素免疫过氧化物酶法可获得最佳结果，该方法还能鉴定石蜡包埋组织中未检测到的抗谷氨酸脱羧酶抗体。
