Hoffmann Rolf
Department of Dermatology, Philipp University, Marburg, Germany.
J Investig Dermatol Symp Proc. 2003 Jun;8(1):109-15. doi: 10.1046/j.1523-1747.2003.12183.x.
Hair loss or hair thinning is a common complaint in clinical dermatology, and patients seeking advice for hair loss are not necessarily bald. Because the effects of treatment attempts are hard to measure, there is need for a sensitive tool to monitor hair loss and treatment responses. Such a method must be able to analyze the biologic parameters of hair growth, which are: (i) hair density (n per cm2); (ii) hair diameter (microm); (iii) hair growth rate (mm per day); and (iv) anagen/telogen ratio. Herein we present the TrichoScan as a method that combines epiluminescence microscopy with automatic digital image analysis for the measurement of human, and potentially animal hair, in situ. The TrichoScan is able to analyze all four parameters of hair growth with a so-called intraclass correlation of approximately 91% within the same TrichoScan operator and an intraclass correlation of approximately 97% for different TrichoScan operators. The application of the technique is demonstrated by comparing the hair parameters in individuals without apparent hair loss, men with untreated androgenetic alopecia, and men after treatment with finasteride (1 mg per day). We were able to detect a significant increase in hair counts and cumulative hair thickness 3 and 6 mo after treatment. Advantages of the TrichoScan are that it can be used for clinical studies to compare placebo versus treatment, to compare different capacities of hair growth promoting substances, to study androgenetic alopecia and other forms of diffuse hair loss, and to study the effects of drugs and laser treatment on hypertrichosis and hirsutism.
脱发或头发稀疏是临床皮肤科常见的主诉，寻求脱发建议的患者不一定是秃头。由于治疗尝试的效果难以衡量，因此需要一种灵敏的工具来监测脱发情况和治疗反应。这样一种方法必须能够分析头发生长的生物学参数，这些参数包括：（i）头发密度（每平方厘米的数量）；（ii）头发直径（微米）；（iii）头发生长速度（每天毫米数）；以及（iv）生长期/休止期比例。在此，我们介绍TrichoScan，这是一种将落射荧光显微镜与自动数字图像分析相结合的方法，用于原位测量人类乃至潜在动物的毛发。TrichoScan能够分析头发生长的所有四个参数，在同一位TrichoScan操作员内部，其所谓的组内相关系数约为91%，不同TrichoScan操作员之间的组内相关系数约为97%。通过比较无明显脱发个体、未经治疗的雄激素性脱发男性以及服用非那雄胺（每天1毫克）治疗后的男性的毛发参数，展示了该技术的应用。我们能够检测到治疗后3个月和6个月头发数量和累积头发厚度显著增加TrichoScan的优点在于，它可用于临床研究，以比较安慰剂与治疗效果、比较促进头发生长物质的不同效能、研究雄激素性脱发和其他形式的弥漫性脱发，以及研究药物和激光治疗对多毛症和毛发过多症的影响。
