Amini Mohammad K, Khorasani Jafar H, Khaloo Shokooh S, Tangestaninejad Shahram
Chemistry Department, University of Isfahan, Isfahan 81746-73441, Iran.
Anal Biochem. 2003 Sep 1;320(1):32-8. doi: 10.1016/s0003-2697(03)00355-5.
A chemically modified electrode constructed by incorporating N,N(')-bis(salicylidene)-1,2-phenylenediaminocobalt(II) into carbon-paste matrix was used as a sensitive electrochemical sensor for detection of cysteine. The resulting electrode exhibits catalytic properties for the electrooxidation of cysteine and lowers the overpotential for the oxidation of this compound. The faster rate of electron transfer results in a near-Nernstian behavior of the modified electrode and makes it a suitable potentiometric and voltammetric sensor for the fast and easy determination of cysteine. A linear response in concentration range from approximately 2 microM to 0.01 M was obtained with a detection limit of 1 microM for the potentiometric detection of cysteine. The modified electrode was also used for the amperometric and differential pulse voltammetric determination of cysteine and the results were compared with those of the potentiometric method.
通过将N,N'-双（水杨醛）-1,2-苯二胺钴（II）掺入碳糊基质构建的化学修饰电极被用作检测半胱氨酸的灵敏电化学传感器。所得电极对半胱氨酸的电氧化具有催化性能，并降低了该化合物氧化的过电位。更快的电子转移速率导致修饰电极呈现近能斯特行为，使其成为快速简便测定半胱氨酸的合适电位和伏安传感器。在约2 microM至0.01 M的浓度范围内获得了线性响应，半胱氨酸电位检测的检测限为1 microM。该修饰电极还用于半胱氨酸的安培和差分脉冲伏安测定，并将结果与电位法的结果进行了比较。
