Beyer U, Fleischer A, Kage A, Haueter U, Ehwald R
Disetronic Medical Systems AG, Burgdorf, Switzerland.
Biosens Bioelectron. 2003 Oct 1;18(11):1391-7. doi: 10.1016/s0956-5663(03)00067-8.
The function of the recently described viscometric affinity sensor (VAS), which measures glucose due to its strong effect on the viscosity of a sensitive liquid containing Concanavalin A (ConA) and dextran, was analysed for osmotic and colloid-osmotic effects on the glucose reading. The suction of low- and high-molecular weight osmotica on the membrane of the microdialysis fibre was measured using a membrane osmometer built from the microdialysis probe of the VAS. The reduction of the sensor read-out in blood plasma can be completely explained by a change in small osmotic volume fluxes through the dialysis membrane, which affect the ConA concentration and the viscosity after the flow of the sensitive liquid through the dialysis probe. The measuring error could be prevented by the presence of the polyethylene glycol 6000 at an isotonic concentration in the glucose standard solutions used for sensor calibration.
最近描述的粘度亲和传感器（VAS）通过测量葡萄糖对含有伴刀豆球蛋白A（ConA）和葡聚糖的敏感液体粘度的强烈影响来检测葡萄糖。分析了渗透压和胶体渗透压对葡萄糖读数的影响。使用由VAS的微透析探头构建的膜渗透压计测量低分子量和高分子量渗透剂对微透析纤维膜的抽吸作用。血浆中传感器读数的降低可以完全由通过透析膜的小渗透体积通量的变化来解释，这会影响敏感液体流过透析探头后的ConA浓度和粘度。在用于传感器校准的葡萄糖标准溶液中加入等渗浓度的聚乙二醇6000可以防止测量误差。
