Ohkawa J, Yuyama N, Taira K
Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
Nucleic Acids Symp Ser. 1992(27):15-6.
We have constructed a "shot-gun" type ribozyme-trimming system. By concatenating several units, each consisting of a trans-acting ribozyme (targeted to HIV-RNA) and cis-acting ribozymes (trimming 5'- and 3'-ends of the trans-acting ribozyme), several kinds of trans-acting ribozymes can be liberated upon transcription and self-cleavage. Since each liberated HIV-RNA-targeted ribozymes can work independently, they can simultaneously cleave HIV-RNA at several different sites. Ribozymes were targeted at relatively conserved GUC-containing sites at LTR, gag and tat regions.
我们构建了一种“霰弹枪”式核酶修剪系统。通过连接几个单元，每个单元由一个反式作用核酶（靶向HIV-RNA）和顺式作用核酶（修剪反式作用核酶的5'和3'末端）组成，几种反式作用核酶在转录和自我切割后可被释放。由于每个释放的靶向HIV-RNA的核酶都能独立发挥作用，它们可以在几个不同位点同时切割HIV-RNA。核酶靶向LTR、gag和tat区域中相对保守的含GUC位点。
