Li J, Jin H, Zhang Q, Song R, Xu Z
National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences.
Chin J Biotechnol. 1995;11(1):27-35.
Two di-component ribozymes RZ34 and RZ13, the simplest forms of multi-component ribozymes, by ligating mono-ribozymes RZ3 and RZ4 and RZ1 and RZ3 were constructed, respectively. The in vitro results indicate that each di-component ribozyme is able to cleave two different TMV transcripts, with the specificity and efficiency similar to their mono-ribozyme counterparts. Possible effects on the specificity and efficiency by temperature and the positions of the component-ribozymes in these di-component ribozymes were also studied.
