Carputo D, Terra A, Barone A, Esposito F, Fogliano V, Monti L, Frusciante L
Department of Soil, Plant and Environmental Sciences (DISSPA), University of Naples Federico II, Via Università 100, 80055 Portici, Italy.
Theor Appl Genet. 2003 Nov;107(7):1187-94. doi: 10.1007/s00122-003-1356-3. Epub 2003 Jul 29.
F(1) and backcross hybrids between sexually incompatible species Solanum commersonii and Solanum tuberosum were characterized for glycoalkaloid content and capacity to cold acclimate. Glycoalkaloid (GA) analysis revealed that F(1) triploids and BC(1) pentaploids contained the glycoalkaloids of both parents. In BC(2) (near) tetraploids the situation was different, in that some hybrids produced the GAs of both parents, whereas others contained only the GAs of S. tuberosum. This suggested that the GAs from S. commersonii may be lost rapidly, and that they may have a simple genetic control. The total tuber GA content of BC(1) and BC(2) groups averaged quite acceptable levels (165.9 mg/kg in BC(1) and 192.8 mg/kg in BC(2)), with six genotypes having a GA content <200 mg/kg fresh weight. The F(1) triploid hybrids expressed a capacity to cold acclimate similar to S. commersonii, whereas BC(1) and BC(2) genotypes generally displayed an acclimation capacity higher than the sensitive parent but lower than S. commersonii. However, one BC(1) and two BC(2) genotypes with an acclimation capacity as high as S. commersonii were identified. The polar lipid fatty acid composition in S. commersonii and its hybrid derivatives showed that, following acclimation, there was a significant increase in 18:3. Correlation analysis between the capacity to cold acclimate and the increase in 18:3 was significant, suggesting that the increase in 18:3 can be used as a biochemical marker for the assisted selection of cold-acclimating genotypes in segregating populations.
对有性不亲和的物种野生番茄（Solanum commersonii）和马铃薯（Solanum tuberosum）之间的F1代及回交杂种进行了糖苷生物碱含量和冷驯化能力的表征。糖苷生物碱（GA）分析表明，F1代三倍体和BC1代五倍体含有双亲的糖苷生物碱。在BC2代（近）四倍体中情况有所不同，一些杂种产生了双亲的GA，而另一些仅含有马铃薯的GA。这表明来自野生番茄的GA可能会迅速丢失，并且它们可能受简单的遗传控制。BC1代和BC2代群体的块茎总GA含量平均处于相当可接受的水平（BC1代为165.9毫克/千克，BC2代为192.8毫克/千克），有6个基因型的GA含量<200毫克/千克鲜重。F1代三倍体杂种表现出与野生番茄相似的冷驯化能力，而BC1代和BC2代基因型通常表现出比敏感亲本更高但比野生番茄低的驯化能力。然而，鉴定出了一个驯化能力与野生番茄一样高的BC1代基因型和两个BC2代基因型。野生番茄及其杂种衍生物的极性脂质脂肪酸组成表明，驯化后18:3有显著增加。冷驯化能力与18:3增加之间的相关性分析具有显著性，这表明18:3的增加可作为在分离群体中辅助选择冷驯化基因型的生化标记。
