Department of Soil, Plant, Environmental, and Animal Production Sciences (DISSPAPA), University of Naples Federico II, Portici (Na), Italy.
Chem Biodivers. 2011 Dec;8(12):2226-37. doi: 10.1002/cbdv.201100038.
The main aim of this work was to study the leaf secondary metabolite profiles of artificially induced tetraploids (2n=4x=48) of Solanum commersonii, a diploid (2n=2x=24) wild potato species. The tetraploid genotypes of S. commersonii were produced by oryzalin treatment. Both HPLC-UV and LC/MS analyses revealed that there were no qualitative differences in the metabolite profiles between the diploid S. commersonii and its tetraploids. By contrast, the results showed that the phenylpropanoid content was generally significantly higher in the tetraploids than in the diploid S. commersonii. Concerning the glycoalkaloids (GAs), the results provided evidence that the content of minor GAs (solanidenediol triose, solanidadienol lycotetraose, and solanidenol lycotetraose) was higher in tetraploids than in the diploid progenitor, while the content of major GAs (dehydrodemissine and dehydrocommersonine) was significantly higher in diploid S. commersonii than in its tetraploid genotypes. The results are discussed from the practical perspective of potato biodiversity enhancement.
本工作的主要目的是研究人工诱导的四倍体（2n=4x=48）茄属植物 Commersonii 的叶次生代谢产物谱，茄属植物 Commersonii 是一种二倍体（2n=2x=24）野生马铃薯种。四倍体基因型的茄属植物 Commersonii 通过草甘膦处理产生。HPLC-UV 和 LC/MS 分析均表明，二倍体茄属植物 Commersonii 与其四倍体之间的代谢产物谱没有定性差异。相比之下，结果表明，四倍体中苯丙素的含量普遍明显高于二倍体茄属植物 Commersonii。关于糖苷生物碱（GAs），结果表明，微量 GAs（茄呢醇三糖、茄呢醇六糖和茄呢醇六糖）的含量在四倍体中高于二倍体祖先，而主要 GAs（去氢德米辛和去氢 Commersonine）的含量在二倍体茄属植物 Commersonii 中明显高于其四倍体基因型。从提高马铃薯生物多样性的实际角度讨论了这些结果。
