在链脲佐菌素诱导的 1 型糖尿病相关生殖系统功能障碍和炎症中的保护作用。

Protective Effect of on Streptozotocin-Induced Type I Diabetes-Associated Reproductive System Dysfunction and Inflammation.

机构信息

Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.

ImmunoPharmaLab, Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy.

出版信息

Molecules. 2022 Sep 17;27(18):6075. doi: 10.3390/molecules27186075.

BACKGROUND

Type-one diabetes (T1D), a chronic autoimmune disease with marked inflammatory responses, is associated with infertility complications and implications. Based on the anti-diabetic, antioxidant, and anti-hyperlipidemic potential of (PO), this study aimed to evaluate the protective effect of this plant extract on streptozotocin-induced type-I-diabetes-associated reproductive system dysfunction and inflammation.

METHODS

Male rats were randomly divided into four experimental groups: control, diabetic, and treatment/s (PO extract at 100 or 300 mg/kg/daily). Then food and water consumption, body, testis and epididymis weights, histopathological evaluation, seminiferous tubules diameter, sperm count and motility, glucose levels, sex hormones, and inflammatory and oxidative stress markers were evaluated.

RESULTS

Our results showed that streptozotocin-induced diabetes significantly increased food and water consumption; increased glucose, MDA, TGF-β1, and TNF-α levels; and decreased the seminiferous tubules diameter, sperm count and motility, levels of LH, testosterone, total thiol, VEGF, and SOD activity. Interestingly, PO extract (phytochemically characterized by using liquid chromatography-mass spectrometry to detect bioactive molecules) significantly ameliorated these parameters and histopathological indexes' damage in rats.

CONCLUSION

Even if more preclinical assessments are needed to better characterize the mechanism/s of action, the results of this study will pave the way for the rational use of PO on diabetic-associated clinical complications and implications.

背景

1 型糖尿病（T1D）是一种慢性自身免疫性疾病，伴有明显的炎症反应，与不孕并发症和影响有关。基于（PO）的抗糖尿病、抗氧化和抗高血脂特性，本研究旨在评估这种植物提取物对链脲佐菌素诱导的 1 型糖尿病相关生殖系统功能障碍和炎症的保护作用。

方法

雄性大鼠随机分为四组实验：对照组、糖尿病组和治疗组/（PO 提取物 100 或 300mg/kg/天）。然后评估食物和水的消耗、体重、睾丸和附睾重量、组织病理学评估、精小管直径、精子计数和活力、血糖水平、性激素以及炎症和氧化应激标志物。

结果

我们的结果表明，链脲佐菌素诱导的糖尿病显著增加了食物和水的消耗；增加了血糖、MDA、TGF-β1 和 TNF-α水平；降低了精小管直径、精子计数和活力、LH、睾酮、总巯基、VEGF 和 SOD 活性水平。有趣的是，PO 提取物（通过液相色谱-质谱联用检测生物活性分子对其进行了化学特征分析）显著改善了这些参数和大鼠的组织病理学指标损伤。