Kanyshkova Tat'yana G, Babina Svetlana E, Semenov Dmitry V, Isaeva Natal'ya, Vlassov Alexander V, Neustroev Kirill N, Kul'minskaya Anna A, Buneva Valentina N, Nevinsky Georgy A
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Eur J Biochem. 2003 Aug;270(16):3353-61. doi: 10.1046/j.1432-1033.2003.03715.x.
Lactoferrin (LF) is a Fe3+-binding glycoprotein, first recognized in milk and then in other human epithelial secretions and barrier fluids. Many different functions have been attributed to LF, including protection from iron-induced lipid peroxidation, immunomodulation and cell growth regulation, DNA binding, and transcriptional activation. Its physiological role is still unclear, but it has been suggested to be responsible for primary defense against microbial and viral infection. We present evidence that different subfractions of purified human milk LF possess five different enzyme activities: DNase, RNase, ATPase, phosphatase, and malto-oligosaccharide hydrolysis. LF is the predominant source of these activities in human milk. Some of its catalytically active subfractions are cytotoxic and induce apoptosis. The discovery that LF possesses these activities may help to elucidate its many physiological functions, including its protective role against microbial and viral infection.
乳铁蛋白（LF）是一种结合Fe3+的糖蛋白，最初在牛奶中被发现，随后在其他人体上皮分泌物和屏障液中被发现。LF具有许多不同的功能，包括防止铁诱导的脂质过氧化、免疫调节和细胞生长调节、DNA结合以及转录激活。其生理作用仍不清楚，但有人认为它负责对微生物和病毒感染的初级防御。我们提供的证据表明，纯化的人乳LF的不同亚组分具有五种不同的酶活性：DNA酶、RNA酶、ATP酶、磷酸酶和麦芽寡糖水解酶。LF是人乳中这些活性的主要来源。其一些具有催化活性的亚组分具有细胞毒性并诱导细胞凋亡。LF具有这些活性的发现可能有助于阐明其许多生理功能，包括其对微生物和病毒感染的保护作用。
