Kim S H, Cho K W, Lim S H, Hwang Y H, Ryu H, Oh S H, Seul K H, Jeong G B, Yoon S
Department of Physiology, Jeonbug National University Medical School, Republic of Korea.
Regul Pept. 1992 Dec 11;42(3):153-62. doi: 10.1016/0167-0115(92)90095-c.
Atrial natriuretic peptide (ANP) has been reported to be locally synthesized in the ovary although its physiological roles are still unknown. To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemical properties. Serial dilution curves made with the extracts of pig granulosa cells, their perfusates and follicular fluid were paralleled to the standard curve of ANP. The amount of irANP in the granulosa cell was 2 fg/cell. The total amount of irANP in granulosa cells significantly correlated with the levels of irANP in follicular fluid. Additionally, the total content of irANP in the follicle negatively correlated with the follicular size. On reverse phase HPLC, the major form of irANP in granulosa cells and follicular fluid was high molecular weight but that in perfusate was low molecular weight. In Northern blot analysis, ANP mRNA was detected in the pig granulosa cells. Immunohistochemistry showed ANP prohormone location in granulosa cells of rat ovary. These data strongly suggest that the granulosa cells synthesize and secrete ANP.
据报道，心房利钠肽（ANP）可在卵巢中局部合成，但其生理作用尚不清楚。为了确定卵巢ANP的来源，我们证实了猪颗粒细胞中免疫反应性（ir）ANP的存在和释放，并对其生化特性进行了表征。用猪颗粒细胞提取物、灌注液和卵泡液绘制的系列稀释曲线与ANP标准曲线平行。颗粒细胞中irANP的含量为2 fg/细胞。颗粒细胞中irANP的总量与卵泡液中irANP的水平显著相关。此外，卵泡中irANP的总含量与卵泡大小呈负相关。在反相高效液相色谱分析中，颗粒细胞和卵泡液中irANP的主要形式是高分子量的，但灌注液中的是低分子量的。在Northern印迹分析中，在猪颗粒细胞中检测到了ANP mRNA。免疫组织化学显示大鼠卵巢颗粒细胞中有ANP前体激素定位。这些数据有力地表明颗粒细胞能合成并分泌ANP。
