Ryu H, Cho K W, Kim S H, Kim S Z, Oh S H, Hwang Y H, Lee G Y
Department of Physiology, Medical School, Jeonbug National University, Jeonju, Republic of Korea.
Gen Comp Endocrinol. 1992 Aug;87(2):171-7. doi: 10.1016/0016-6480(92)90019-g.
The presence of immunoreactive atrial natriuretic peptide (irANP) and ANP gene expression in the frog lymph heart was examined by a radioimmunoassay (RIA) combined with HPLC and by Northern blot hybridization of total RNA. Serial dilution curve of the lymph heart extract was paralleled with the RIA standard curve. The lymph heart contained 153.32 +/- 35.80 pg of irANP/mg of wet tissue. The major form of irANP in the frog lymph heart was high molecular weight on reverse-phase and gel permeation high performance liquid chromatography as in the frog atria and ventricles. The frog lymph heart, as well as frog atria and ventricles, was shown to express mRNA coding for ANP. Dense core secretory granules similar to those observed in the mammalian atria were also found in the frog lymph heart. The presence of irANP and the expression of ANP gene in the frog lymph heart suggest that the lymph heart may participate in the regulation of homeostasis of lymph circulation and blood volume change through the synthesis and release of ANP.
采用放射免疫分析法（RIA）结合高效液相色谱法（HPLC）以及对总RNA进行Northern印迹杂交，检测了蛙类淋巴心内免疫反应性心房钠尿肽（irANP）的存在情况以及ANP基因的表达。淋巴心提取物的系列稀释曲线与RIA标准曲线平行。淋巴心每毫克湿组织含153.32±35.80皮克的irANP。与蛙类心房和心室一样，蛙类淋巴心内irANP的主要形式在反相和凝胶渗透高效液相色谱上表现为高分子量。结果显示，蛙类淋巴心以及蛙类心房和心室均表达编码ANP的mRNA。在蛙类淋巴心内还发现了与哺乳动物心房中所见类似的致密核心分泌颗粒。蛙类淋巴心内irANP的存在以及ANP基因的表达表明，淋巴心可能通过合成和释放ANP参与淋巴循环和血容量变化的体内平衡调节。
