Song J Y, Guo S X
Department of Mycology, Institute of Medicinal Plant Development, CAMS, PUMC, Beijing 100094, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Dec;23(6):547-51.
To examine effects of fungus AR-18 (Epulorhiza sp.) on the growth of Dendrobium candidum and D. nobile in vitro culture.
Effects of fungus AR-18 on fresh weight and dry weight of D. candidum and D. nobile were studied in vitro culture, when agar was used as rest, and effects of fungus AR-18 and nutrients on fresh weight and dry weight of D. candidum and D. nobile were studied in vitro culture, when vermiculite was used as rest.
In agar medium, effects of fungus AR-18 on fresh weight and dry weight of D. candidum and D. nobile were not significant (P > 0.05). However, in vermiculite medium, the effect of fungus AR-18 on fresh weight of D. nobile was significant (P < 0.05). Fresh weight of D. nobile inoculated with fungus AR-18 was increased by 16% compared with the non-inoculated ones (control). The effect of fungus AR-18 on dry weight of D. nobile was very significant (P < 0.01). Dry weight of D. nobile inoculated with fungus AR-18 was increased by 21% compared with the control. In vermiculite medium, the effect of combination treatment (Fungus AR-18 + Distilled H2O) on fresh weight of D. nobile was very significant (P < 0.01). Fresh weight of D. nobile inoculated with fungus AR-18 was increased by 47% compared with the control, while the effect of combination treatment (Fungus AR-18 + Nutrients) on fresh weight of D. nobile was not significant (P > 0.05). In addition, the effect of combinations treatment (Fungus AR-18 + Nutrients) and (Fungus AR-18 + Distilled H2O) on dry weight of D. nobile was not significant (P > 0.05). So the combination treatment (Fungus AR-18 + Distilled H2O) was the best for the growth of D. nobile. In vermiculite medium, effects of fungus AR-18 and nutrients on fresh weight and dry weight of D. candidum were not significant (P > 0.05).
It is important in vitro culture to select a proper rest for the control of the growth of fungus to establish a beneficial symbiotic relationship between plants and fungi. If the fungus AR-18 is utilized as biological manure in got-up planting of D. nobile, a good result may be obtained.
研究真菌AR - 18（Epulorhiza sp.）对铁皮石斛和金钗石斛离体培养生长的影响。
以琼脂为基质时，研究真菌AR - 18对铁皮石斛和金钗石斛鲜重及干重的影响；以蛭石为基质时，研究真菌AR - 18和营养物质对铁皮石斛和金钗石斛鲜重及干重的影响。
在琼脂培养基中，真菌AR - 18对铁皮石斛和金钗石斛鲜重及干重的影响不显著（P > 0.05）。然而，在蛭石培养基中，真菌AR - 18对金钗石斛鲜重的影响显著（P < 0.05）。接种真菌AR - 18的金钗石斛鲜重比未接种的（对照）增加了16%。真菌AR - 18对金钗石斛干重的影响极显著（P < 0.01）。接种真菌AR - 18的金钗石斛干重比对照增加了21%。在蛭石培养基中，组合处理（真菌AR - 18 + 蒸馏水）对金钗石斛鲜重的影响极显著（P < 0.01）。接种真菌AR - 18的金钗石斛鲜重比对照增加了47%，而组合处理（真菌AR - 18 + 营养物质）对金钗石斛鲜重的影响不显著（P > 0.05）。此外，组合处理（真菌AR - 18 + 营养物质）和（真菌AR - 18 + 蒸馏水）对金钗石斛干重的影响不显著（P > 0.05）。所以组合处理（真菌AR - 18 + 蒸馏水）对金钗石斛生长最为有利。在蛭石培养基中，真菌AR - 18和营养物质对铁皮石斛鲜重及干重的影响不显著（P > 0.05）。
在离体培养中，选择合适的基质对于控制真菌生长、建立植物与真菌之间有益的共生关系很重要。如果将真菌AR - 18作为生物肥料用于金钗石斛的规模化种植，可能会取得良好效果。
