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四种内生真菌对金钗石斛生长及多糖和生物碱含量的影响

[Effects of four species of endophytic fungi on the growth and polysaccharide and alkaloid contents of Dendrobium nobile].

作者信息

Chen Xiao-mei, Guo Shun-xing

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Bejing 100094, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2005 Feb;30(4):253-7.

PMID:15724397
Abstract

OBJECTIVE

To study the effects of four species of endophytic fungi on the growth and polysaccharide and alkaloid contents of cultured Dendrobium nobile.

METHOD

Each fungal strain was cultured together with D. nobile, and the plant weight as well as the polysaccharide and alkaloid contents were measured.

RESULT

Compared with the control group, Mycena sp. (MF23) was found to decrease the fresh weight of D. nobile by 24.9% (P < 0.01). All four species of fungi have no effect on the dry weight of this plant. On the other hand, Epulorhiza sp. (MF18) and MF23 could increase the percent of dry weight of seedlings (P < 0.05). Epulorhiza sp. (MF15) and MF24 increased the number of aerial roots by 4.25 times and 4.14 times respectively (P < 0.01). MF23 decreased the numbers of basal roots by 46.5% (P < 0.01). MF15, MF18, MF23 and MF24 were shown to increase the content of polysacchride by 153.4%, 52.1%, 18.5% and 76.7%, respectively. MF23 also increased the content of total alkaloid by 18.3%.

CONCLUSION

Endophytic fungi cultured together with D. nobile could affect the growth of this plant; they also increase total alkaloid and polysaccharide contents.

摘要

目的

研究四种内生真菌对组培金钗石斛生长及多糖和生物碱含量的影响。

方法

将每种真菌菌株与金钗石斛共同培养,测定植株重量以及多糖和生物碱含量。

结果

与对照组相比,发现 Mycena sp.(MF23)使金钗石斛鲜重降低了24.9%(P < 0.01)。所有四种真菌对该植物干重均无影响。另一方面,Epulorhiza sp.(MF18)和MF23可提高幼苗干重百分比(P < 0.05)。Epulorhiza sp.(MF15)和MF24使气生根数量分别增加了4.25倍和4.14倍(P < 0.01)。MF23使基生根数量减少了46.5%(P < 0.01)。MF15、MF18、MF23和MF24分别使多糖含量提高了153.4%、52.1%、18.5%和76.7%。MF23还使总生物碱含量提高了18.3%。

结论

与金钗石斛共同培养的内生真菌可影响该植物的生长;它们还能提高总生物碱和多糖含量。

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Zhongguo Zhong Yao Za Zhi. 2005 Feb;30(4):253-7.
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