[一种可产生紫杉醇类化合物的内生真菌的分离]
[Isolation of a kind of endophytic fungus which can produce taxol compounds].
作者信息
Shao A J, Lin S F, Zhang S J, Li X M, Huang L Q, Chen M, Wu Z G, Dai R Q, Liu L
机构信息
Department of Pharmacognosy, Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Dec;23(6):642-4.
OBJECTIVE
To isolate a kind of endophytic fungus which can produce taxol compounds from the bark of Taxus Cuspidata Sieb et Zucc.
METHODS
Endophytic fungus was isolated from the bark of Taxus Cuspidata Sieb et Zucc by aseptic techniques and the analysis of the culture fluid from the fermentation of fungus was carried out by high performance liquid chromatography (HPLC).
RESULTS
Endophytic fungus was obtained from the bark of Taxus Cuspidata Sieb et Zucc and the chemical analysis showed that the culture of fungus contain taxol compounds.
CONCLUSION
Endophytic fungus which can produce taxol compounds exists in the bark of Taxus Cuspidata Sieb et Zucc.
目的
从东北红豆杉树皮中分离出一种能产生紫杉醇类化合物的内生真菌。
方法
采用无菌技术从东北红豆杉树皮中分离内生真菌，并通过高效液相色谱法（HPLC）对真菌发酵培养液进行分析。
结果
从东北红豆杉树皮中获得了内生真菌，化学分析表明该真菌培养物中含有紫杉醇类化合物。
结论
东北红豆杉树皮中存在能产生紫杉醇类化合物的内生真菌。
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