Zhang Peng, Zhou Peng-Peng, Yu Long-Jiang
Key Laboratory of Molecular Biophysics Ministry of Education, Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
FEMS Microbiol Lett. 2009 Apr;293(2):155-9. doi: 10.1111/j.1574-6968.2009.01481.x. Epub 2009 Feb 23.
An endophytic taxol-producing fungus (strain MD3) isolated from the inner bark of Taxus x media was identified as Aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18S rRNA gene sequence analysis. Taxol produced by A. candidus MD3 was shown to be identical to authentic taxol analyzed by UV, HPLC, MS and nuclear magnetic resonance spectroscopy. The gene encoding the 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the taxol biosynthetic pathway, has been cloned from A. candidus MD3 for the first time and possesses high homology to the same gene found in Taxus spp.
从杂种红豆杉（Taxus x media）内皮中分离出的一株产紫杉醇内生真菌（菌株MD3），根据其形态特征、生理生化特征及18S rRNA基因序列分析，被鉴定为白曲霉（Aspergillus candidus）。通过紫外光谱、高效液相色谱、质谱和核磁共振光谱分析表明，白曲霉MD3产生的紫杉醇与标准紫杉醇相同。首次从白曲霉MD3中克隆出编码10-去乙酰巴卡亭III-10-O-乙酰基转移酶的基因，该酶催化紫杉醇生物合成途径中最后一个二萜中间体的形成，且与红豆杉属中发现的同一基因具有高度同源性。
