基于从红豆杉进行PCR扩增筛选产紫杉醇的真菌。
Screening of Taxol-producing fungi based on PCR amplification from Taxus.
作者信息
Zhang Peng, Zhou Peng-Peng, Jiang Chen, Yu Hui, Yu Long-Jiang
机构信息
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
出版信息
Biotechnol Lett. 2008 Dec;30(12):2119-23. doi: 10.1007/s10529-008-9801-7. Epub 2008 Aug 17.
Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.
编码10 - 去乙酰巴卡亭III - 10 - O - 乙酰转移酶和C - 13苯丙烷侧链 - CoA酰基转移酶的基因被用作筛选产紫杉醇内生真菌的分子标记。通过聚合酶链反应（PCR），从南方红豆杉和云南红豆杉中分离出的90株内生真菌中有3株得到阳性结果。这3个菌株在300毫升马铃薯/葡萄糖液体培养基中于25℃培养10天时，每克干重菌丝体中含有100 - 160微克紫杉醇。
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