Koyama Yoshiyuki, Ito Tomoko, Matsumoto Hidetoshi, Tanioka Akihiko, Okuda Tatsuya, Yamaura Nobuaki, Aoyagi Haruhiko, Niidome Takuro
Department of Home Economics, Otsuma Women's University, Chiyoda-ku, Tokyo 102-8357, Japan.
J Biomater Sci Polym Ed. 2003;14(6):515-31. doi: 10.1163/15685620360674227.
Novel carboxylic acid pendant-containing poly(ethylene glycol) (PEG) derivatives (PEG-Cs) were synthesized by the chemical modification of the carbon-carbon double-bond side chain of copoly(allyl glycidyl ether/ethylene oxide) (copoly(AGE/EO)). PEG-C showed a protecting ability for the DNA/polycation complex from the albumin-induced aggregation. It also expressed the enhanced efficiency on the polycation-mediated gene transfection on the cultured cells (up to 3-4-times higher), probably due to its disperse-stabilizing property and also the proton-sponge effect.
通过对共聚（烯丙基缩水甘油醚/环氧乙烷）（共聚（AGE/EO））的碳-碳双键侧链进行化学修饰，合成了新型含羧酸侧基的聚乙二醇（PEG）衍生物（PEG-Cs）。PEG-C对DNA/聚阳离子复合物具有保护作用，可防止白蛋白诱导的聚集。它在培养细胞上的聚阳离子介导的基因转染效率也有所提高（高达3-4倍），这可能归因于其分散稳定特性以及质子海绵效应。
