• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Efficient gene delivery system mediated by cis-aconitate-modified chitosan-g-stearic acid micelles.

作者信息

Yao Jing-Jing, Du Yong-Zhong, Yuan Hong, You Jian, Hu Fu-Qiang

机构信息

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People's Republic of China.

出版信息

Int J Nanomedicine. 2014 Jun 18;9:2993-3003. doi: 10.2147/IJN.S61103. eCollection 2014.

DOI:10.2147/IJN.S61103
PMID:24971010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4069129/
Abstract

Cis-aconitate-modified chitosan-g-stearic acid (CA-CSO-SA) micelles were synthesized in this study to improve the gene transfection efficiency of chitosan-g-stearic acid (CSO-SA). The CA-CSO-SA micelles had a similar size, critical micelle concentration, and morphology, but their zeta potential and cytotoxicity were reduced compared with CSO-SA micelles. After modification with cis-aconitate, the CA-CSO-SA micelles could also compact plasmid DNA (pDNA) to form nanocomplexes. However, the DNA binding ability of CA-CSO-SA was slightly reduced compared with that of CSO-SA. The transfection efficiency mediated by CA-CSO-SA/pDNA against HEK-293 cells reached up to 37%, and was much higher than that of CSO-SA/pDNA (16%). Although the cis-aconitate modification reduced cellular uptake kinetics in the initial stages, the total amount of cellular uptake tended to be the same after 24 hours of incubation. An endocytosis inhibition experiment showed that the internalization mechanism of CA-CSO-SA/pDNA in HEK-293 cells was mainly via clathrin-mediated endocytosis, as well as caveolae-mediated endocytosis and macropinocytosis. Observation of intracellular trafficking indicated that the CSO-SA/pDNA complexes were trapped in endolysosomes, but CA-CSO-SA/pDNA was more widely distributed in the cytosol. This study suggests that modification with cis-aconitate improves the transfection efficiency of CSO-SA/pDNA.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/44ec0cdc4d00/ijn-9-2993Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/a1f24d3fbd5e/ijn-9-2993Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/5dc2e2907604/ijn-9-2993Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/1c750f7973a4/ijn-9-2993Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/56cc83ade1d2/ijn-9-2993Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/4f49764aab29/ijn-9-2993Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/e7147ad2dce1/ijn-9-2993Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/44ec0cdc4d00/ijn-9-2993Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/a1f24d3fbd5e/ijn-9-2993Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/5dc2e2907604/ijn-9-2993Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/1c750f7973a4/ijn-9-2993Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/56cc83ade1d2/ijn-9-2993Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/4f49764aab29/ijn-9-2993Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/e7147ad2dce1/ijn-9-2993Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73be/4069129/44ec0cdc4d00/ijn-9-2993Fig7.jpg

相似文献

1
Efficient gene delivery system mediated by cis-aconitate-modified chitosan-g-stearic acid micelles.
Int J Nanomedicine. 2014 Jun 18;9:2993-3003. doi: 10.2147/IJN.S61103. eCollection 2014.
2
Receptor-mediated gene delivery by folic acid-modified stearic acid-grafted chitosan micelles.叶酸修饰的硬脂酸接枝壳聚糖胶束介导的受体基因传递。
Int J Nanomedicine. 2011;6:1559-68. doi: 10.2147/IJN.S23828. Epub 2011 Aug 1.
3
A novel chitosan oligosaccharide-stearic acid micelles for gene delivery: properties and in vitro transfection studies.一种用于基因递送的新型壳寡糖-硬脂酸胶束：性质及体外转染研究
Int J Pharm. 2006 Jun 6;315(1-2):158-66. doi: 10.1016/j.ijpharm.2006.02.026. Epub 2006 Mar 6.
4
Synthesis of lamivudine stearate and antiviral activity of stearic acid-g-chitosan oligosaccharide polymeric micelles delivery system.硬脂酸酯化拉米夫定的合成及硬脂酸-g-壳聚糖寡糖聚合物胶束给药系统的抗病毒活性。
Eur J Pharm Sci. 2010 Nov 20;41(3-4):498-507. doi: 10.1016/j.ejps.2010.08.004. Epub 2010 Aug 20.
5
Synthesis and antitumor activity of doxorubicin conjugated stearic acid-g-chitosan oligosaccharide polymeric micelles.阿霉素偶联硬脂酸-g-壳聚糖寡糖聚合物胶束的合成及抗肿瘤活性。
Biomaterials. 2009 Dec;30(36):6955-63. doi: 10.1016/j.biomaterials.2009.09.008. Epub 2009 Sep 25.
6
Cellular uptake and cytotoxicity of shell crosslinked stearic acid-grafted chitosan oligosaccharide micelles encapsulating doxorubicin.包载阿霉素的壳交联硬脂酸接枝壳寡糖胶束的细胞摄取及细胞毒性
Eur J Pharm Biopharm. 2008 May;69(1):117-25. doi: 10.1016/j.ejpb.2007.09.018. Epub 2007 Oct 5.
7
Enhanced cellular uptake of chlorine e6 mediated by stearic acid-grafted chitosan oligosaccharide micelles.硬脂酸接枝壳寡糖胶束介导的氯e6细胞摄取增强
J Drug Target. 2009 Jun;17(5):384-91. doi: 10.1080/10611860902894325.
8
Quaternary complexes composed of plasmid DNA/protamine/fish sperm DNA/stearic acid grafted chitosan oligosaccharide micelles for gene delivery.由质粒 DNA/鱼精蛋白/鱼精 DNA/硬脂酸接枝壳聚糖低聚糖胶束组成的季铵复合物用于基因传递。
Int J Biol Macromol. 2011 Jan 1;48(1):153-9. doi: 10.1016/j.ijbiomac.2010.10.011. Epub 2010 Oct 28.
9
Effect of anionic PEGylated polypeptide on gene transfection mediated by glycolipid conjugate micelles.阴离子聚乙二醇化多肽对糖脂共轭胶束介导的基因转染的影响。
Mol Pharm. 2015 Apr 6;12(4):1072-83. doi: 10.1021/mp500560h. Epub 2015 Mar 10.
10
Redox-responsive polymer-drug conjugates based on doxorubicin and chitosan oligosaccharide-g-stearic acid for cancer therapy.基于阿霉素和壳寡糖-g-硬脂酸的氧化还原响应性聚合物-药物偶联物用于癌症治疗。
Mol Pharm. 2015 Apr 6;12(4):1193-202. doi: 10.1021/mp500710x. Epub 2015 Mar 16.

引用本文的文献

1
Recent advances of metal-based nanoparticles in nucleic acid delivery for therapeutic applications.金属基纳米颗粒在核酸治疗应用中的最新进展。
J Nanobiotechnology. 2022 Nov 24;20(1):501. doi: 10.1186/s12951-022-01650-z.
2
Hepatitis B virus S gene therapy with 10-23 DNAzyme delivered by chitosan--stearic acid micelles.壳聚糖-硬脂酸胶束递送10-23脱氧核酶的乙肝病毒S基因治疗
RSC Adv. 2019 May 15;9(27):15196-15204. doi: 10.1039/c9ra00330d. eCollection 2019 May 14.
3
Endocytosis in gene therapy with non-viral vectors.非病毒载体基因治疗中的内吞作用。

本文引用的文献

1
Overcoming nonviral gene delivery barriers: perspective and future.克服非病毒基因递送障碍：现状与未来。
Mol Pharm. 2013 Nov 4;10(11):4082-98. doi: 10.1021/mp400467x. Epub 2013 Oct 16.
2
Effect of proteins with different isoelectric points on the gene transfection efficiency mediated by stearic acid grafted chitosan oligosaccharide micelles.不同等电点蛋白质对硬脂酸接枝壳寡糖胶束介导的基因转染效率的影响
Mol Pharm. 2013 Jul 1;10(7):2568-77. doi: 10.1021/mp300732d. Epub 2013 May 29.
3
The effect of crosslinking agents on the transfection efficiency, cellular and intracellular processing of DNA/polymer nanocomplexes.
Wien Med Wochenschr. 2016 May;166(7-8):227-35. doi: 10.1007/s10354-016-0450-5. Epub 2016 May 3.
4
The janus facet of nanomaterials.纳米材料的双面性。
Biomed Res Int. 2015;2015:317184. doi: 10.1155/2015/317184. Epub 2015 May 17.
交联剂对 DNA/聚合物纳米复合物的转染效率、细胞内和细胞内处理的影响。
Biomaterials. 2013 Apr;34(13):3479-88. doi: 10.1016/j.biomaterials.2013.01.072. Epub 2013 Feb 8.
4
Effective antitumor gene therapy delivered by polyethylenimine-conjugated stearic acid-g-chitosan oligosaccharide micelles.聚亚乙基亚胺-硬脂酸-g-壳聚糖寡糖胶束介导的有效抗肿瘤基因治疗。
Gene Ther. 2013 Jun;20(6):597-606. doi: 10.1038/gt.2012.72. Epub 2012 Sep 6.
5
Serum tolerance and endosomal escape capacity of histidine-modified pDNA-loaded complexes based on polyamidoamine dendrimer derivatives.基于聚酰胺-胺树枝状大分子衍生物的组氨酸修饰 pDNA 负载复合物的血清耐受性和内涵体逃逸能力。
Biomaterials. 2012 Nov;33(32):8111-21. doi: 10.1016/j.biomaterials.2012.07.032. Epub 2012 Aug 14.
6
Influence of the polyanion on the physico-chemical properties and biological activities of polyanion/DNA/polycation ternary polyplexes.多阴离子对聚阴离子/DNA/聚阳离子三元聚集体的理化性质和生物活性的影响。
Acta Biomater. 2012 Aug;8(8):3014-26. doi: 10.1016/j.actbio.2012.04.034. Epub 2012 Apr 27.
7
Rational design of smart supramolecular assemblies for gene delivery: chemical challenges in the creation of artificial viruses.智能超分子组装体用于基因传递的合理设计：人工病毒构建中的化学挑战。
Chem Soc Rev. 2012 Apr 7;41(7):2562-74. doi: 10.1039/c1cs15258k. Epub 2011 Nov 21.
8
Polysaccharides as building blocks for nanotherapeutics.多糖作为纳米治疗药物的构建模块。
Chem Soc Rev. 2012 Apr 7;41(7):2623-40. doi: 10.1039/c1cs15239d. Epub 2011 Nov 15.
9
Effects of hydrophobic and hydrophilic modifications on gene delivery of amphiphilic chitosan based nanocarriers.疏水和亲水修饰对两亲性壳聚糖纳米载体基因传递的影响。
Biomaterials. 2011 Jul;32(20):4630-8. doi: 10.1016/j.biomaterials.2011.03.003. Epub 2011 Mar 25.
10
Chitosan and its derivatives for gene delivery.壳聚糖及其衍生物在基因传递中的应用。
Int J Biol Macromol. 2011 Mar 1;48(2):234-8. doi: 10.1016/j.ijbiomac.2010.11.013. Epub 2010 Dec 4.