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包裹于磷酸钙中的DNA/金纳米颗粒的制备

Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate.

作者信息

Ito Tomoko, Ibe Koyuki, Uchino Tomohiro, Ohshima Hiroyuki, Otsuka Makoto

机构信息

Research Institute of Pharmaceutical Sciences, Musashino University, Shinmachi 1-1-20, Nishitokyo-Shi, Tokyo 202-8585, Japan.

出版信息

J Drug Deliv. 2011;2011:647631. doi: 10.1155/2011/647631. Epub 2011 Jun 7.

DOI:10.1155/2011/647631
PMID:21773047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3134889/
Abstract

Biocompatible DNA/gold nanoparticle complex with a protective calcium phosphate (CaP) coating was prepared by incubating DNA/gold nanoparticle complex coated by hyaluronic acid in SBF (simulated body fluid) with a Ca concentration above 2 mM. The CaP-coated DNA complex was revealed to have high compatibility with cells and resistance against enzymatic degradation. By immersion in acetate buffer (pH 4.5), the CaP capsule released the contained DNA complex. This CaP capsule including a DNA complex is promising as a sustained-release system of DNA complexes for gene therapy.

摘要

通过将包被有透明质酸的DNA/金纳米颗粒复合物在钙离子浓度高于2 mM的SBF（模拟体液）中孵育，制备了具有保护性磷酸钙（CaP）涂层的生物相容性DNA/金纳米颗粒复合物。结果表明，CaP包被的DNA复合物与细胞具有高度相容性，并且具有抗酶降解能力。通过浸入醋酸盐缓冲液（pH 4.5）中，CaP胶囊释放出其中所含的DNA复合物。这种包含DNA复合物的CaP胶囊有望作为用于基因治疗的DNA复合物的缓释系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/4f7f3f9bacc4/JDD2011-647631.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/b5bdb7aa37ed/JDD2011-647631.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/f7121d1a1137/JDD2011-647631.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/b3bc74932238/JDD2011-647631.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/9bb601733961/JDD2011-647631.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/2c90f597a526/JDD2011-647631.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/90721c9edd15/JDD2011-647631.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/3fc8c0f456ae/JDD2011-647631.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/4f7f3f9bacc4/JDD2011-647631.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/b5bdb7aa37ed/JDD2011-647631.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/f7121d1a1137/JDD2011-647631.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/b3bc74932238/JDD2011-647631.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/9bb601733961/JDD2011-647631.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/2c90f597a526/JDD2011-647631.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/90721c9edd15/JDD2011-647631.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/3fc8c0f456ae/JDD2011-647631.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cc7/3134889/4f7f3f9bacc4/JDD2011-647631.008.jpg

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