Huang Jian-Qiang, Xie Can-Mao
Department of Respirology, The First Affiliated Hospital, Zhongshan University, Guangzhou 510080, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Apr;26(4):206-9.
To investigate the effects of erythromycin on secretion of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta(1) (TGF-beta(1)), and the level of connexin 43 in human pleural mesothelial cells (HPMC), and to explore the mechanism of pleurodesis.
HPMC was incubated with erythromycin at concentrations of 100 mg/L or 25 mg/L. The levels of TNF-alpha and TGF-beta(1) in the supernatants were measured by ELISA method, and levels of connexin 43 were detected by Western blot.
The secretion of TNF-alpha by HMPC increased after incubation with 100 mg/L erythromycin for 3 or 5 days, and the secretion of TGF-beta(1) increased markedly after incubation with lower or higher concentrations of erythromycin. The levels of connexin 43 in HPMC decreased after stimulation with 100 mg/L erythromycin, but no relationship was observed between the levels and the stimulation time.
HPMC incubated with erythromycin showed increased secretion of TNF-alpha and TGF-beta(1), which may be one of the mechanisms for erythromycin pleurodesis. Erythromycin decreased the level of connexin 43 in HPMC, and this effect may be a response of the cells to the stimulus. A high concentration of erythromycin is suggested for clinical pleurodesis based on the results from this study.
研究红霉素对人胸膜间皮细胞（HPMC）肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）分泌及连接蛋白43水平的影响，探讨胸膜固定术的机制。
将HPMC分别与浓度为100mg/L或25mg/L的红霉素孵育。采用酶联免疫吸附测定法（ELISA法）检测上清液中TNF-α和TGF-β1的水平，采用蛋白质免疫印迹法检测连接蛋白43的水平。
100mg/L红霉素孵育HMPC 3天或5天后，TNF-α分泌增加；低浓度或高浓度红霉素孵育后，TGF-β1分泌均显著增加。100mg/L红霉素刺激后，HPMC中连接蛋白43水平降低，但该水平与刺激时间无关。
红霉素孵育的HPMC中TNF-α和TGF-β1分泌增加，这可能是红霉素胸膜固定术的机制之一。红霉素降低了HPMC中连接蛋白43的水平，这种作用可能是细胞对刺激的反应。基于本研究结果，建议临床胸膜固定术采用高浓度红霉素。
