• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

红霉素对人胸膜间皮细胞活力、肿瘤坏死因子-α和转化生长因子-β1分泌及连接蛋白43表达的影响。

The effects of erythromycin on the viability and the secretion of TNF-alpha and TGF-beta1 and expression of connexin43 by human pleural mesothelial cells.

作者信息

Xie Canmao, Huang Jian Qiang, Light Richard W

机构信息

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhongshan (Sun Yat-sen) University, Guangzhou, China.

出版信息

Respirology. 2005 Nov;10(5):567-71. doi: 10.1111/j.1440-1843.2005.00751.x.

DOI:10.1111/j.1440-1843.2005.00751.x
PMID:16268908
Abstract

OBJECTIVE

The mechanism by which erythromycin produces pleurodesis remains unknown. The purpose of this study was to investigate the effects of erythromycin on human pleural mesothelial cell (HPMC) viability, the secretion of tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta(1) (TGF-beta(1)) and the level of expression of connexin43.

METHODOLOGY

HPMC were incubated with different concentrations of erythromycin. The inhibitory effects of erythromycin on HPMC growth were measured using a tetrazolium-based colorimetric assay. The levels of TNF-alpha and TGF-beta(1) in supernatants were measured by ELISA and levels of connexin43 were assessed by Western blot.

RESULTS

Erythromycin injured HPMC in a dose and time-dependent manner. The secretion of both TNF-alpha and TGF-beta(1) by HMPC increased significantly when they were incubated with 100 mg/L erythromycin for 3 or 5 days. The levels of connexin43 in HPMC decreased after incubation with 100 mg/L erythromycin and no relationship was observed between the levels and incubation time.

CONCLUSIONS

Erythromycin injures HPMC in a dose- and time-dependent manner and results in the secretion of TNF-alpha and TGF-beta(1). This is one possible mechanism of pleurodesis with erythromycin. Furthermore, erythromycin decreased the levels of connexin43 in HPMC, which could possibly affect the response of HPMC to pleurodesis with erythromycin.

摘要

目的

红霉素产生胸膜固定术的机制尚不清楚。本研究的目的是探讨红霉素对人胸膜间皮细胞（HPMC）活力、肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）分泌以及连接蛋白43表达水平的影响。

方法

将HPMC与不同浓度的红霉素孵育。采用基于四氮唑的比色法测定红霉素对HPMC生长的抑制作用。通过酶联免疫吸附测定法（ELISA）测量上清液中TNF-α和TGF-β1的水平，并通过蛋白质印迹法评估连接蛋白43的水平。

结果

红霉素以剂量和时间依赖性方式损伤HPMC。当HMPC与100mg/L红霉素孵育3天或5天时，TNF-α和TGF-β1的分泌均显著增加。与100mg/L红霉素孵育后，HPMC中连接蛋白43的水平降低，且未观察到该水平与孵育时间之间的关系。

结论

红霉素以剂量和时间依赖性方式损伤HPMC，并导致TNF-α和TGF-β1的分泌。这是红霉素胸膜固定术的一种可能机制。此外，红霉素降低了HPMC中连接蛋白43的水平，这可能会影响HPMC对红霉素胸膜固定术的反应。

相似文献

1
The effects of erythromycin on the viability and the secretion of TNF-alpha and TGF-beta1 and expression of connexin43 by human pleural mesothelial cells.红霉素对人胸膜间皮细胞活力、肿瘤坏死因子-α和转化生长因子-β1分泌及连接蛋白43表达的影响。
Respirology. 2005 Nov;10(5):567-71. doi: 10.1111/j.1440-1843.2005.00751.x.
2
[The effects of erythromycin on the secretion of tumor necrosis factor-alpha and transforming growth factor-beta1 and expression of connexin 43 in human pleural mesothelial cells].[红霉素对人胸膜间皮细胞肿瘤坏死因子-α和转化生长因子-β1分泌及连接蛋白43表达的影响]
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Apr;26(4):206-9.
3
Troglitazone inhibits synthesis of transforming growth factor-beta1 and reduces matrix production in human peritoneal mesothelial cells.曲格列酮可抑制转化生长因子-β1的合成，并减少人腹膜间皮细胞中的基质产生。
Nephrology (Carlton). 2006 Dec;11(6):516-23. doi: 10.1111/j.1440-1797.2006.00654.x.
4
[Interactions of ovarian carcinoma cells and human peritoneal mesothelial cells involved in matrix metalloproteinases expressions of ovarian carcinoma cells].[卵巢癌细胞与人腹膜间皮细胞的相互作用涉及卵巢癌细胞基质金属蛋白酶的表达]
Zhonghua Fu Chan Ke Za Zhi. 2006 Mar;41(3):194-7.
5
Tumor necrosis factor alpha enhances secretion of transforming growth factor beta2 in MCF-7 breast cancer cells.肿瘤坏死因子α增强MCF-7乳腺癌细胞中转化生长因子β2的分泌。
Clin Cancer Res. 1996 May;2(5):827-35.
6
[Cytokine-induced release of plasminogen activator inhibitor-1 by human pleural mesothelial cells].[细胞因子诱导人胸膜间皮细胞释放纤溶酶原激活物抑制剂-1]
Zhonghua Nei Ke Za Zhi. 2000 Sep;39(9):599-602.
7
Cytokines regulate matrix metalloproteinases in human uterine endometrial fibroblast cells through a mechanism that does not involve increases in extracellular matrix metalloproteinase inducer.细胞因子通过一种不涉及细胞外基质金属蛋白酶诱导剂增加的机制来调节人子宫内膜成纤维细胞中的基质金属蛋白酶。
Am J Reprod Immunol. 2006 Sep;56(3):201-14. doi: 10.1111/j.1600-0897.2006.00418.x.
8
Overexpression of regucalcin suppresses cell response for tumor necrosis factor-alpha or transforming growth factor-beta1 in cloned normal rat kidney proximal tubular epithelial NRK52E cells.调节钙素的过表达抑制克隆的正常大鼠肾近端小管上皮NRK52E细胞对肿瘤坏死因子-α或转化生长因子-β1的细胞反应。
J Cell Biochem. 2007 Apr 1;100(5):1178-90. doi: 10.1002/jcb.21105.
9
A selective cyclooxygenase-2 inhibitor decreases transforming growth factor-beta1 synthesis and matrix production in human peritoneal mesothelial cells.一种选择性环氧化酶-2抑制剂可降低人腹膜间皮细胞中转化生长因子-β1的合成及基质产生。
Cell Biol Int. 2007 May;31(5):508-15. doi: 10.1016/j.cellbi.2006.11.018. Epub 2006 Nov 28.
10
TGF-beta and TNF-alpha producing effects of losartan and amlodipine on human mononuclear cell culture.氯沙坦和氨氯地平对人单核细胞培养物产生转化生长因子-β和肿瘤坏死因子-α的作用。
Nephrology (Carlton). 2005 Oct;10(5):478-82. doi: 10.1111/j.1440-1797.2005.00462.x.

引用本文的文献

1
Erythromycin poudrage versus erythromycin slurry in the treatment of refractory spontaneous pneumothorax.红霉素撒布剂与红霉素悬液治疗难治性自发性气胸的比较
J Thorac Dis. 2018 Feb;10(2):757-765. doi: 10.21037/jtd.2018.01.48.
2
TNF-alpha inhibits asbestos-induced cytotoxicity via a NF-kappaB-dependent pathway, a possible mechanism for asbestos-induced oncogenesis.肿瘤坏死因子-α通过核因子-κB依赖途径抑制石棉诱导的细胞毒性，这可能是石棉诱导肿瘤发生的一种机制。
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10397-10402. doi: 10.1073/pnas.0604008103. Epub 2006 Jun 23.