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分子分析在寄生虫流行病学中的重要性及陷阱

Importance and pitfalls of molecular analysis to parasite epidemiology.

作者信息

Constantine Clare C

机构信息

Western Australian Biomedical Research Institute, Division of Health Science, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch WA 6150, Australia.

出版信息

Trends Parasitol. 2003 Aug;19(8):346-8. doi: 10.1016/s1471-4922(03)00167-3.

Abstract

Molecular tools are increasingly being used to address questions about parasite epidemiology. Parasites represent a diverse group and they might not fit traditional population genetic models. Testing hypotheses depends equally on correct sampling, appropriate tool and/or marker choice, appropriate analysis and careful interpretation. All methods of analysis make assumptions which, if violated, make the results invalid. Some guidelines to avoid common pitfalls are offered here.

摘要

分子工具越来越多地被用于解决有关寄生虫流行病学的问题。寄生虫种类繁多,可能不符合传统的群体遗传模型。检验假设同样取决于正确的抽样、合适的工具和/或标记选择、恰当的分析以及审慎的解读。所有分析方法都有假设条件,若这些条件被违反,结果就会无效。本文提供了一些避免常见错误的指导原则。

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