Constantine Clare C
Western Australian Biomedical Research Institute, Division of Health Science, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch WA 6150, Australia.
Trends Parasitol. 2003 Aug;19(8):346-8. doi: 10.1016/s1471-4922(03)00167-3.
Molecular tools are increasingly being used to address questions about parasite epidemiology. Parasites represent a diverse group and they might not fit traditional population genetic models. Testing hypotheses depends equally on correct sampling, appropriate tool and/or marker choice, appropriate analysis and careful interpretation. All methods of analysis make assumptions which, if violated, make the results invalid. Some guidelines to avoid common pitfalls are offered here.
分子工具越来越多地被用于解决有关寄生虫流行病学的问题。寄生虫种类繁多,可能不符合传统的群体遗传模型。检验假设同样取决于正确的抽样、合适的工具和/或标记选择、恰当的分析以及审慎的解读。所有分析方法都有假设条件,若这些条件被违反,结果就会无效。本文提供了一些避免常见错误的指导原则。