伊朗西部洛雷斯坦省动物物种分离株的分子特征分析

Molecular Characterization of Animal Spp. Isolates from Lorestan Province, Western Iran.

作者信息

Heydarian Peyman, Jajarmi Vahid, Spotin Adel, Ashrafi Keyhan, Mohebali Mehdi, Aryaeipour Mojgan, Bozorgomid Arezoo, Hajialilo Elham, Javad Abbaszadeh Afshar Mohammad, Fadaei Tehrani Mandana, Bagher Rokni Mohammad

机构信息

Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Public Health. 2022 Aug;51(8):1847-1856. doi: 10.18502/ijph.v51i8.10271.

DOI:10.18502/ijph.v51i8.10271

PMID:36249111

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9546809/

Abstract

BACKGROUND

We aimed to detect the genetic diversity of samples identified morphologically as spp. from sheep, cattle and goat from Lorestan Province, western Iran using PCR-RFLP method. Besides, we evaluated the genetic diversity indices, sequencing and phylogenetic analysis using mitochondrial gene ( and ).

METHODS

PCR-RFLP analysis of ribosomal fragment by restriction enzyme to investigate the genetic characteristics of species obtained from different hosts (18 sheep, 21 cattle, and 17goats) was conducted. The samples were sequenced. Sequences were evaluated using BLAST software and the parasite species were identified with similarity percentage and overlap with the species registered in the gene bank. Then similarity and diversity of intra-species and intra-species diversity of species were calculated.

RESULTS

In Lorestan, based on RFLP pattern, 93% (52) of the spp. isolates had a RFLP pattern related to and 7% (4) were . No hybrid forms were detected. The gene could clarify 19 haplotypes against gene that found 22 haplotypes among livestock. Sequencing results of the mtDNA showed intra-species identity 98. 5%-100% and Intra-species-diversity: 0-1.5% compared to the GenBank sequences.

CONCLUSION

Using PCR-RFLP method, two species of and , were present in Lorestan Province, but was more prevalent. Mitochondrial genes could better test variability indices in different hosts than ribosomal genes, consequently among mitochondrial genes, the ND1 gene could better examine differences and similarities than CO1.

摘要

背景

我们旨在使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，检测从伊朗西部洛雷斯坦省的绵羊、牛和山羊身上形态学鉴定为某物种的样本的遗传多样性。此外，我们使用线粒体基因（某基因1和某基因2）评估了遗传多样性指数、测序及系统发育分析。

方法

通过某限制性内切酶对核糖体某片段进行PCR-RFLP分析，以研究从不同宿主（18只绵羊、21头牛和17只山羊）获得的该物种的遗传特征。对样本进行测序。使用BLAST软件评估序列，并根据相似百分比以及与基因库中登记物种的重叠情况鉴定寄生虫物种。然后计算该物种的种内相似性和多样性以及种内多样性。

结果

在洛雷斯坦，基于RFLP模式，该物种分离株中有93%（52个）具有与某物种相关的RFLP模式，7%（4个）为另一物种。未检测到杂交形式。与某基因相比，某基因可明确19种单倍型，而某基因在牲畜中发现了22种单倍型。与GenBank序列相比，线粒体DNA的测序结果显示种内同一性为98.5%-100%，种内多样性为0-1.5%。