[利用针对p24蛋白的单克隆抗体对HIV-1抗原进行免疫酶检测]
[The immunoenzyme detection of the HIV-1 antigen by using monoclonal antibodies to protein p24].
作者信息
Shumaĭ E P, Vorob'ev S M, Makarova N E, Tugizov Sh M, Zverev V V, Kushch A A
出版信息
Vopr Virusol. 1992 Sep-Dec;37(5-6):229-32.
PMID:1290220
Abstract
A test system using monoclonal antibodies to HIV p24 was developed for solid-phase ELISA for detection of HIV antigen (AG) which helped detect the content of AG in samples with trace concentrations, less than 25 pg/ml. The test system can be used for AG monitoring in natural specimens (serum of HIV-infected patients and culture medium of infected cells) and for determination of antigen-recombinant product of HIV gag gene.
摘要
开发了一种使用抗HIV p24单克隆抗体的检测系统，用于固相ELISA检测HIV抗原（AG），该系统有助于检测浓度低于25 pg/ml的痕量样品中AG的含量。该检测系统可用于天然标本（HIV感染患者的血清和感染细胞的培养基）中的AG监测以及HIV gag基因抗原重组产物的测定。
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